In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first su...

Sodium dodecyl sulfate (SDS) is one of the most commonly used detergent in house holds and in Industry. It is a component of a number of industrially useful products. After use, like all other xenobiotics, it is discharged in water bodies in huge amounts. It is now realized that it is toxic to fishes and to animals. In this review, we have made an attempt to compile the data regarding the toxic...

Synthesis of human placental lactogen (hPL) and of human chorionic gonadotropin (hCG) by membrane-bound and free polyribosomes from early and from full term human placentas was investigated by in vitro release of the nascent hormone peptides, followed by immunoprecipitation and electrophoresis in sodium dodecyl sulfate gels, and by specific binding of 125I-labeled hPL antibody to nascent peptid...

We have developed a simple immunoaffinity chromatography procedure for the purification of a glycosyl-phosphatidylinositol (GPI)-specific phospholipase D (GPI-PLD) from bovine serum. The enzyme was initially purified by a procedure consisting of 9% polyethylene glycol precipitation, Q Sepharose anion-exchange chromatography, S-300 gel filtration, wheat germ lectin-Sepharose, hydroxylapatite aga...

Rational: Smoking kills more than one billion current smokers worldwide prematurely due to various tobacco-related diseases. Smoking contributes to multiple well-documented adverse health effects, including heart disease, pulmonary disease and lung and other cancers in both industrialized and developing countries. Tobacco smoking is a leading cause of preventable disease and death. If the curre...