Mutants of Salmonella typhimurium that lack the biosynthetic sulfite reductase (cysI and cysJ mutants) retain the ability to reduce sulfite for growth under anaerobic conditions (E. L. Barrett and G. W. Chang, J. Gen. Microbiol., 115:513-516, 1979). Here we report studies of sulfite reduction by a cysI mutant of S. typhimurium and purification of the associated anaerobic sulfite reductase. Sulf...

Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differ...

By comparing the batches of UMAGIS medium prepared in our laboratory in the years 1953 to 1954, it was observed that the slow H2S formers such as Salmonella typhosa gave poor H2S formation. Since all the compounds except the sulphur containing ones are stable, an attempt was made to reactivate the medium by the addition of freshly prepared sodium sulphite and sodium thiosulphate. The results we...

The study of the depression effect non-toxic depressants on flotation separation chalcopyrite from galena is great importance for both industrial applications and theoretical research. mixed depressant (DFinal) four common inhibitors—sodium carboxymethyl cellulose, sodium silicate, sulfite, zinc sulfate—exhibited high selectivity during galena. Flotation tests an copper–lead bulk concentrate sh...

Exposure to (bi)sulfite (HSO3-) and sulfite (SO32-) has been shown to induce a wide range of adverse reactions in sensitive individuals. Studies have shown that peroxidase-catalyzed oxidation of (bi)sulfite leads to formation of several reactive free radicals, such as sulfur trioxide anion (.SO3-), peroxymonosulfate (-O3SOO.), and especially the sulfate (SO4. -) anion radicals. One such peroxid...

In general, the meat contains nutrients of high biological value and susceptible to contamination by enteropathogens. One way minimizing/eliminating this is use food additives, in case meat, usually sodium sulphite. This technological resource used inhibit growth pathogenic bacteria such as Salmonella spp. thermotolerant coliforms, mandatory parameters foreseen sanitary legislation. Therefore, ...

Sulfur dioxide and other so-called sulfiting agents as additives in foods and drugs may cause severe allergic reactions in susceptible individuals, especially asthmatics. The most widely used chemical preservatives, they have become a subject of increasing concern since 1976. The issue was recently brought to national attention by a CBS "60 Minutes" report on sulfite sensitivity aired twice in ...