Fetal bovine and dog serum were selectively freed of plasminogen by affinity chromatography. The resulting serum as well as native and reconstituted serum (obtained by the addition of purified plasminogen to the plasminogen-depleted serum) were used to examine the role of plasminogen in (a) growth of normal and SV-40-transformed hamster embryo fibroblasts in liquid medium, (b) growth of SV-40-t...

Multi-wavelength fluorescence spectroscopy was evaluated in this work as tool for real-time monitoring of antibody aggregation in CHO fed-batch cultivations via partial least square (PLS) modeling. Therefore, we used the extrinsic fluorescence dyes 1-anilinonaphthalene-8-sulfonate (ANS), 4,4'-bis-1-anilinonaphthalene-8-sulfonate (Bis-ANS), or Thioflavin T (ThT) as medium additives. This is a ne...

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 µM)-induced platelet aggregation (ca 80% inhibi...

the concept of fuzzy soft γ-ring is introduced; and some properties of fuzzy soft γ-rings are given. then the definitions of fuzzy soft γ-ideals are proposed and some of their theories are considered.

We consider a multi-agent scenario where a collection of agents needs to select a common decision from a large set of decisions over which they express their preferences. This decision set has a combinatorial structure, that is, each decision is an element of the Cartesian product of the domains of some variables. Agents express their preferences over the decisions via soft constraints. We cons...

Risk is one of the major factors to decide the software cost, reliability and the software dead lines. As the software proposal is presented along with this risk analysis also begin. With each step of software development some risk factor is added with software cost. Other then this some other factors like availability of resources, software duration also effect the software risk. In this propo...

We propose an algorithm for the hierarchical aggregation of observations in dissemination-based, distributed traffic information systems. Instead of transmitting observed parameters directly, we propose soft-state sketches—an extension of FlajoletMartin sketches—as a probabilistic approximation. This data representation is duplicate insensitive, a trait that overcomes two central problems of ex...

In this paper we propose wireless sensor network architecture with layered protocols, targeting different aspects of the awareness requirements in wireless sensor networks. Under such a unified framework, we pay special attention to the most important awareness issues in wireless sensor networks: namely dynamic awareness, energy awareness and spatiotemporal awareness. First, we propose the spat...

background: protein aggregation is one of the important, common and troubling problems in biotechnology, pharmaceutical industries and amyloid-related disorders. methods: in the present study, the inhibitory effects of some carbohydrates (alginate, β-cyclodextrin and trehalose) on the formation of nano-globular aggregates from normal (hsa) and glycated (ghsa) human serum albumin were studied; w...

Savignygrin, a platelet aggregation inhibitor that possesses the RGD integrin recognition motif, has been purified from the soft tick Ornithodoros savignyi. Two isoforms with similar biological activities differ because of R52G and N60G in their amino acid sequences, indicating a recent gene duplication event. Platelet aggregation induced by ADP (IC50, 130 nm), collagen, the thrombin receptor-a...