The effect of cultivation parameters such as temperature incubation, IPTG induction and ethanol shock on the production of Pseudomonas aeruginosa amidase (E.C.3.5.1.4) in a recombinant Escherichia coli strain in LB ampicillin culture medium was investigated. The highest yield of soluble amidase, relatively to other proteins, was obtained in the condition at 37°C using 0.40 mM IPTG to induce gro...

Insufficient nutrient and pest infection are two major predicaments for low crop yield in agriculture. Nematodes, a most infectious parasite pest in plants responsible for major yield loss. Chemical phosphate fertilizer made large reserves of it in soil, but part of it is made available to the plants because of irreversible fixation of it with divalent cation. In the present study, Arthrobotrys...

Many root-colonizing pseudomonads are able to promote plant growth by increasing phosphate availability in soil through solubilization of poorly soluble rock phosphates. The major mechanism of phosphate solubilization by pseudomonads is the secretion of gluconic acid, which requires the enzyme glucose dehydrogenase and its cofactor pyrroloquinoline quinone (PQQ). The main aim of this study was ...

The rabbit intestinal sucrase-isomaltase complex has been purified to homogeneity after solubilization with Triton X 100 followed by chromatography on DEAE Sepharose CL 6B and a second solubilization with papain. After hydrophobic chromatography on Octyl Sepharose CL 6B, separation from other contaminating maltases was achieved by gel filtration on Ultrogel ACA 22. The final enzyme was purified...

The acid stress chaperones HdeA and HdeB of Escherichia coli prevent the aggregation of periplasmic proteins at acidic pH. We show in this report that they also form mixed aggregates with proteins that have failed to be solubilized at acidic pH and allow their subsequent solubilization at neutral pH. HdeA, HdeB, and HdeA and HdeB together display an increasing efficiency for the solubilization ...

Plants acquire phosphorus from soil solution as phosphate anion. It is the least mobile element in plant and soil contrary to other macronutrients. It precipitates in soil as orthophosphate or is absorbed by Fe and Al oxides through legend exchange. Phosphorus solubilizing bacteria play role in phosphorus nutrition by enhancing its availability to plants through release from inorganic and organ...

We provide a validated and rapid protocol for the solubilization of amyloid β-peptide (Aβ). This procedure involves sequential solubilization using structure-breaking organic solvents hexafluoroisopropanol and DMSO followed by column purification. The low solubility and tendency of Aβ to aggregate considerably impede the in vitro handling and biophysical or biological investigation of Aβ, despi...

The mechanisms of action of mineral phosphate solubilization (MPS) were studied in the wild-type Mps‡ Penicillium rugulosum strain IR94-MF1 and in negative (Mps3) and superpositive (Mps‡‡) mutants derived from it. MPS activities were measured in liquid media using sucrose as C source, four N (arginine, nitrate, nitrate+ammonium and ammonium) and P sources (KH2PO4, hydroxyapatite, FePO4 and AlPO...

The outer membrane fraction from Rhodopseudomonas sphaeroides was isolated by isopycnic density centrifugation. The purity of this fraction was assayed by several methods. When the outer membrane fraction obtained after French press lysis of cells was compared with the outer membrane fragments released during spheroplast formation, the polypeptide profiles were identical. Detergent solubilizati...

A technologically and economically feasible process called bioleaching was used for the removal of heavy metals from livestock sludge with indigenous sulfur-oxidizing bacteria in this study. The effects of sludge solids concentration on the bioleaching process were examined in a batch bioreactor. Due to the buffering capacity of sludge solids, the rates of pH reduction, ORP rise and metal solub...