High fecundity, rapid generation time, and external development of optically clear embryos make the zebrafish (Danio rerio) a convenient vertebrate model for genetic, developmental, and disease studies. Efficient sperm cryopreservation enhances the zebrafish model system by optimizing productive use of facility space, extending the reproductive lifetime of males, providing an alternative to liv...

BACKGROUND Cryopreservation of human semen for assisted reproduction is complicated by cryodamage to spermatozoa caused by excessive reactive oxygen species (ROS) generation. METHODS AND FINDINGS We used exogenous ROS (H(2)O(2)) to simulate cryopreservation and examined DNA damage repair in embryos fertilized with sperm with H(2)O(2)-induced DNA damage. Sperm samples were collected from epidi...

Submit Manuscript | http://medcraveonline.com reduced [3]. The reduction in fertility is documented in patients following orchidectomy with a contralateral viable testicle. In this case the contralateral testicle was atrophic following previous misdiagnosed torsion. Patients with severe testicular atrophy have undergone successful microdissection of sperm, in one study up to 56% of patients wit...

Submit Manuscript | http://medcraveonline.com reduced [3]. The reduction in fertility is documented in patients following orchidectomy with a contralateral viable testicle. In this case the contralateral testicle was atrophic following previous misdiagnosed torsion. Patients with severe testicular atrophy have undergone successful microdissection of sperm, in one study up to 56% of patients wit...

Carbohydrates are candidates for the basis of species-selective interaction of gametes during mammalian fertilization. In this study, we sought to clarify the roles of sugar residues in the species-selective, sperm-oocyte interaction in pigs and cattle. Acrosome-intact porcine and bovine sperm exhibited their strongest binding affinities for β-Gal and α-Man residues, respectively. Porcine-sperm...

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at differen...

While there have been several relatively recent comprehensive reviews of mammalian sperm cryopreservation (Watson, 2001; Leibo, 2004), this chapter is not meant to be a review of the literature of mammalian sperm cryopreservation. Instead, we intend to provide an understanding of the history, current status, and potential future direction of mammalian sperm cryobiology. There are many reasons w...

The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analys...

Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples. While the overall selectivities of many lectins have been characterized, such studies generally require individually purified lecti...

We used fracture-label and surface labeling techniques to characterize the distribution and topology of wheat germ agglutinin (WGA) receptors in the plasma membrane of boar sperm heads. We show that freeze-fracture results in preferential, but not exclusive, partition of WGA-binding sites with the outer (exoplasmic) half of the plasma membrane. Labeling of the inner (protoplasmic) half of the m...