نتایج جستجو برای: sphere formation assay
تعداد نتایج: 769670 فیلتر نتایج به سال:
Complexation reactions of environmentally important inorganic anions such as nitrate, chloride, sulfate, arsenate, and phosphate on the surface of hydrotalcite-like compounds (HT) were investigated to understand the role of HT in the immobilization of hazardous anions in an alkaline environment. The effects of surface complexation reactions on the solid state properties of HT were also evaluate...
We have reexamined the similarity solution for a self-gravitating isothermal gas sphere and examined implication to star formation in a turbulent cloud. When parameters are adequately chosen, the similarity solution expresses an accreting isothermal gas sphere bounded by a spherical shock wave. The mass and radius of the sphere increases in proportion to the time, while the central density decr...
PURPOSE To demonstrate the presence of corneal stromal precursors that express neural markers in vitro. METHODS To isolate sphere-forming cells, human corneal stromal cells were subjected to a reaggregation-free neurosphere assay in medium containing methylcellulose gel matrix. To promote differentiation, the isolated sphere colonies were plated in wells with medium containing fetal bovine se...
Aldehyde dehydrogenase 1 (ALDH1) is a cancer stem-like cell (CSC) marker in human cancers; however, the specific ALDH1regulated function and its underlying signalling pathways have not been fully demonstrated. Here, we investigated the ALDH1-regulated function and its underlying signalling and tested whether all-trans retinoic acid (ATRA) can suppress ALDH1-regulated tumour behaviour in ovarian...
Methods CSCs were isolated using the sphere-forming assay in serum-free medium under non-adherent conditions and characterized for the expression of mesenchymal stem cell markers (CD90, CD105, CD73) by flow cytometry. The chemosensitity of MNNG and CSCs to doxorubicin (DOX), cisplatin (CIS) and methotrexate (MTX) was evaluated using the MTT colorimetric assay after an incubation period of 48h. ...
Stratified flow past a three-dimensional obstacle such as a sphere has been a long-lasting subject of geophysical, environmental and engineering fluid dynamics. In order to investigate the effect of the stratification on the near wake, in particular, the unsteady vortex formation behind a sphere, numerical simulations of stratified flows past a sphere are conducted. The time-dependent NavierSto...
after the soviet union dissolution, a chaotic period was begun in the russia. russia lost its glory and felt disgrace. the first group of elites came to power under yeltsin; they tried to re-define russia’s identity as a european country and build a foreign policy on this baseline. therefore russia tried to become closer with the west especially with the u.s. according to their view the sovie...
We analyze the structural formation of a polymer chain inside of an attractive sphere depending on the attraction strength. Our model is composed of a coarse-grained polymer and an attractive sphere potential. Within this frame, multicanonical Monte Carlo simulations are employed to identify the global minimum energies for a polymer chain interacting with the attractive inner wall of the sphere...
BACKGROUND Novel therapies tailored to the molecular composition of esophageal squamous cell carcinoma (ESCC) are needed to improve patient survival. We investigated the regulatory network of long intergenic non-protein coding RNA, regulator of reprogramming (linc-ROR) and sex-determining region Y-box 9 (SOX9), and their therapeutic relevance in ESCC. METHODS Linc-ROR and SOX9 expression were...
MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not been fully elucidated. It is necessary to clarify the function of miR-92a-3p in glioma and GSCs to deve...
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