نتایج جستجو برای: standard pcr
تعداد نتایج: 683958 فیلتر نتایج به سال:
BACKGROUND Quantification of integrated proviral HIV DNA by repetitive-sampling Alu-HIV PCR is a candidate virological tool to monitor the HIV reservoir in patients. However, the experimental procedures and data analysis of the assay are complex and hinder its widespread use. Here, we provide an improved and simplified data analysis method by adopting binomial and Poisson statistics. METHODS ...
The recent outbreaks of West Nile virus (WNV) in the northeastern United States and other regions of the world have made it essential to develop an efficient protocol for surveillance of WNV. In the present report, we describe a high-throughput procedure that combines automated RNA extraction, amplification, and detection of WNV RNA. The procedure analyzed 96 samples in approximately 4.5 h. A r...
comparative evaluation of pcr and standard serological methods in detection of brucellosis in cattle
brusellosis is one of the most dangerous infectious zoonotic diseases which at present affect most areas of iran directly or indirectly.the aim of the present study was evaluate the efficacy of pcr test in detection of brucellosis. suspected animal sera (n=20) to brusellosis were collected from clinics and subjected to rose bengal and wright agglutination tests and to pcr analysis. while 6 samp...
background: among the most important parasitic disease, causing diarrhea, giardia lamblia is noteworthy. nowadays detection methods for these parasites include parasitological methods such as microscopic examination. the sensitivity of these methods relies on the expertise and experience of examiners. in contrast, molecular methods such as pcr are less dependent on the expertise of the exami...
We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has bee...
Dermatophyte fungi are a common cause of skin, nail and hair infections globally, ranging from mild to cosmetically disfiguring, or even invasive in rare cases. Specimens requiring fungal microscopy culture for suspected dermatophyte infection make up significant portion the workload diagnostic microbiology laboratories. Whilst still considered gold standard, culture-based method is labour inte...
Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simult...
Competitive PCR (cPCR) is a quantitative PCR approach based on the addition of an internal standard to the PCR mixture. In this study, cPCR was used to quantitate genetically tagged cyanobacteria in Baltic Sea sediment. The cyanobacterium Synechocystis 6803-luc has a chromosomal insertion of the firefly luciferase gene, luc, as a marker detectable by PCR. A competitive standard was constructed ...
We have developed a quantitative competitive PCR (QC-PCR) assay in a microplate format for quantifying human immunodeficiency virus Type 1 (HIV-1) DNA or RNA in a broad range of source materials. Our QC-PCR assay is a modification of technique originally described by Piatak et al. (1993), which is based on the presence of a competitive internal standard containing an internal 80-bp deletion of ...
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