In order to study the bond-slip constitutive model between prestressed tendons and concrete, pull-out tests of single-strand multi-strands specimens are carried out. The effect number on failure mode, slip characteristics concrete strain analyzed, models for single- strand multi-strand also proposed. results show that bond mechanism steel is basically similar deformed bar, but process along axi...

Network security protocols make use of cryptographic techniques to achieve goals such as confidentiality, authentication, integrity and non-repudiation. However, the fact that strong cryptographic algorithms exist does not guarantee the security of a communications system. In fact, it is recognised that the engineering of security protocols is a very challenging task, since protocols that appea...

For a long time, NMR chemical shifts have been used to identify protein secondary structures. Currently, this is accomplished through comparing the observed (1)H(alpha), (13)C(alpha), (13)C(beta), or (13)C' chemical shifts with the random coil values. Here, we present a new protocol, which is based on the joint probability of each of the three secondary structural types (beta-strand, alpha-heli...

The emergence of NextGen sequencing technology has generated much interest in the exploration of transcriptomes. Currently, Illumina Inc. (San Diego, CA) provides one of the most widely utilized sequencing platforms for gene expression analysis. While Illumina reagents and protocols perform adequately in RNA-sequencing (RNA-seq), alternative reagents and protocols promise a higher throughput at...

DNA polymerase is essential for replication and repair. As it only performs the 5?-3? polymerization, there are two kinds of replication. One them called strand-displacement synthesis: opens double-strand (ds) to attain 3?-5?strand (leading strand) copy this template in a continuous way, other extension copies newly separated strand (lagging discontinuous manner. The complex T7 phage an optimal...

The flow field, tracer dispersion and uniformity of strands in two designs four-strand tun-dishes under normal conditions single-strand blockage are studied by numerical simulation. casting speed (flow rate) increasing uniformly or non-uniformly to improve the strand condition. cases evaluated a novel outflow percentage analysis method. results show that field tundish does not change significan...

Early tenorrhaphy mobilization increases repair site strength and decreases adhesions. Preliminary unpublished data suggest that early active mobilization improves clinical outcome compared with traditional passive motion protocols. We loaded cadaver flexor profundus tendon repairs to 8.0 kg (78.4 N) for up to 5,000 cycles to simulate the loads and cycle number of our active flexor tendon rehab...

Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial transcription and replication imaging protocol), that reveals simultaneously mtDNA and RNA, and that can also be coupled to immunofluorescence for in...

PREMISE OF THE STUDY An efficient alternative strategy to conventional cloning was needed to generate high-quality DNA sequences from a variety of nuclear orthologs for phylogenetic studies. This method would facilitate studies and minimize technical problems typically encountered in cloning methodologies. METHODS We tested a variety of single-strand conformation polymorphism (SSCP) protocols...

The logic BAN was developed in the late eighties to reason about authenticated key establishment protocols. It uncovered many aws and properties of protocols, thus generating lots of attention in protocol analysis. BAN itself was also subject of much attention, and work was done examining its properties and limitations, developing extensions and alternatives, and giving it a semantics. More rec...