L-Lactate dehydrogenase from Bifidobacterium longum shows homotropic activation by pyruvate as well as heterotropic activation by fructose 1,6-bisphosphate. Hybrid enzymes were produced from the wild-type subunit and a mutant subunit, whose substrate specificity was altered to that of malate dehydrogenase, and separated to analyze the substrate-induced homotropic activation mechanism. Oxamate, ...

The folding of ribosomal RNAs is central to the biogenesis mitoribosome and a complex, stepwise process. Five recent cryo-EM studies detail late steps maturation human mitoribosomal large subunit RNA that forms catalytic core ribosome: peptidyl transferase center (PTC).

millions of diarrheal disease is made by enterotoxigenic e. coli (etec) each year, specifically in developing countries. in the pathogenesis of etec infections, the first phase is sticking of the bacterium to the minute intestinal epithelium, as a result of colonization factors (cfs) mediation and subsequently generate enterotoxins. these cfs in accordance with their structure are diverged into...

Integrins are unusual signalling proteins that function to signal both from the extracellular environment into cell, but also cytoplasm external of cell. The intracellular cascades associated with integrin activation focus on protein kinase activities, such as focal adhesion and Src. Based this association between signals activity, we have chosen include integrins in 'Catalytic receptors' secti...

Pleosporaceae is an important Dothideomycetes family. To elucidate relationships among some selected anamorphic pleosporalean taxa, their Internal Transcribed Spacer (ITS) and RNA polymerase second largest subunit (RPB2) were sequenced and compared. Phylogenetic analyses of both ITS and RPB2 regions were almost similar and generally congruent with previously described phylogenies and morphology...

The point cloud is the basis for 3D object surface reconstruction. An incomplete significantly reduces accuracy of downstream work such as reconstruction and recognition. Therefore, point-cloud repair indispensable work. However, original shape difficult to restore due uncertainty position new filling point. Considering advantages convex set in dealing with problems, we propose a method via tha...

Background: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a...

The amino acid sequence deduced from cDNA of the human placental fibronectin receptor is reported. The receptor is composed of two subunits: an alpha subunit of 1,008 amino acids which is processed into two polypeptides disulfide bonded to one another, and a beta subunit of 778 amino acids. Each subunit has near its COOH terminus a hydrophobic segment. This and other sequence features suggest a...

Chronic exposure to nicotine, as occurs during tobacco smoking, is one of several factors that have been reported to cause an upregulation of neuronal nicotinic acetylcholine receptors (nAChRs). Here, the influence of both chronic exposure to nicotine (10 microm, 24 hr) and the coexpression of subunit chimeras has been examined in cultured cell lines expressing recombinant alpha4beta2 nAChRs, a...