Networks of interacting protein control physiological processes in all living cells. Considerable effort has recently been invested in understanding protein interactions under normal and diseased conditions. One approach to elucidate the composition of protein complexes is native fractionation followed by immunological or MS-based identification of individual compounds. Native fractionation, in...

Objective(s) Herpes simplex viruses (HSVs) have widespread and ubiquitous prevalence in the human population and they have received a great deal of attention due to the range of diseases, they caused as a result of an infection. It seems that the fast and reliable diagnostic methods are needed for detecting the herpes simplex virus type 1 (HSV1) antibodies especially in patients with HSV encep...

Manire, G. P. (Institute for Virus Research, Kyoto University, Kyoto, Japan). Structure of purified cell walls of dense forms of meningopneumonitis organisms. J. Bacteriol. 91:409-413. 1966.-Purified suspensions of the dense form of the meningopneumonitis organism were prepared by differential centrifugation, sonic treatment, enzyme treatment, and sucrose gradient centrifugation. When mixed wit...

The binding of [3H]benzo(a)pyrene to proteins of rat liver cytosol, the nuclear uptake of cytosolic protein-bound [3H]-benzo(a)pyrene, and the subsequent nuclear metabolism of the polycyclic hydrocarbon were investigated. The binding of [3H]benzo(a)pyrene to cytosol had a saturable high affinity component with a Kd of 2.54 nM and a capacity of 530 fmol/mg protein. Specific binding of [3H]benzo(...

In normal human kidney from adult males cytoplasmic components which bound 17beta-estradiol specifically and with high affinity were demonstrated by dextran-coated charcoal assay, sucrose gradient centrifugation and agar gel electrophoresis. The dissociation constant of the oestradiol-binder complex amounted to 2.2 +/- 0.1 x 10(-9) mol/l. The binding capacity was limited to 34.0 +/- 9.7 fmol/mg...

A double antibody sandwich (DAS) enzyme-linked immunosorbent assay (ELISA) was developed to quantify 146s antigen of foot-and-mouth disease virus (FMDV) strain Al 0 Holland grown in suspension cultures of surviving bovine tongue epithelium. When virus harvests were incubated with trypsin-which affects VP1 , the most immunogenic structural protein of FMDV-the concentration of 146s antigen as det...

1. A method is described for the sucrose-gradient sedimentation analysis of ribosomes in a post-mitochondrial supernatant of rat skeletal muscle. 2. An essential feature of the method involves the use of buffer of ionic strength 0.3 for homogenization of the muscle tissue. 3. Polyribosomes can be prepared by precipitation from post-mitochondrial supernatant of skeletal muscle by adjustment of t...

Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible biological role in bone formation. We have utilized competition studies, saturation analysis, sucrose-density-gradient sedimentation and DEAE-cellulose chromatography to demonstrate that long bones of vitamin D-depleted newborn rats contain cytoplasmic and possibly nuclear receptors that bind 24,25-dih...

Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification of EVs. EVs in bulk were isolated from pooled normal human amniotic fluid (AF) by differential centrifugation followed by IEC or sucrose densi...

When niacin-requiring E. coli was starved of niacin, a precursor of nicotinamide adenine dinucleotide (NAD), the cells elongated and synthesis of DNA was inhibited while syntheses of RNA and protein continued. The cellular NAD concentration decreased to less than one tenth of the normal level in niacin deficiency, but no change in DNA ligase level was detected. The apparent inhibition of DNA sy...