BACKGROUND Recent efforts demonstrated the potential application of cyanobacteria as a "microbial cell factory" to produce butanol directly from CO2. However, cyanobacteria have very low tolerance to the toxic butanol, which limits the economic viability of this renewable system. RESULTS Through a long-term experimental evolution process, we achieved a 150% increase of the butanol tolerance i...

Running head: Carbon regulation in Synechocystis 1 2 correspondence should be sent to: 3 4 Martin Hagemann 5 address: Plant Physiology Department, Institute of Biological Sciences, University of 6 Rostock, Albert-Einstein-Strasse 3, D-18059 Rostock, Germany 7 phone: +49 (0)381-498-6113 8 email: [email protected] 9 10 Research areas: Biochemistry and Metabolism, Cell Biology, Signal...

Nucleotide sequence of the psbK gene of the cyanobacterium Synechocystis 6803 The psbK gene is found in chloroplast, cyanelle and cyanobacterial genomes (1-3). It encodes a low molecular weight photosystem H-associated polypeptide (1). This gene is closely linked to the psbl gene in chloroplast genomes. We have previously reported the isolation and restriction mapping of a Synechocystis 6803 AE...

UNLABELLED Polyamines are found in all groups of cyanobacteria, but their role in environmental adaptation has been barely investigated. In Synechocystis sp. strain PCC 6803, inactivation of spermidine synthesis genes significantly reduced the survivability under chill (5°C)-light stress, and the survivability could be restored by addition of spermidine. To analyze the effects of spermidine on ...

A mutation was recovered in the slr0721 gene, which encodes the decarboxylating NADP(+)-dependent malic enzyme in the cyanobacterium Synechocystis sp. strain PCC 6803, yielding the mutant 3WEZ. Under continuous light, 3WEZ exhibits poor photoautotrophic growth while growing photoheterotrophically on glucose at rates nearly indistinguishable from wild-type rates. Interestingly, under diurnal lig...

The ntcA gene from Synechococcus sp. strain PCC 7942 encodes a regulatory protein which is required for the expression of all of the genes known to be subject to repression by ammonium in that cyanobacterium. Homologs to ntcA have now been cloned by hybridization from the cyanobacteria Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120. Sequence analysis has shown that these ntc...

Ketones are a class of important organic compounds. As the simplest ketone, acetone is widely used as solvents or precursors for industrial chemicals. Presently, million tonnes of acetone is produced worldwide annually, from petrochemical processes. Here we report a biotechnological process that can produce acetone from CO(2), by designing and creating a modularized synthetic pathway in enginee...

UNLABELLED Synechocystis sp. strain PCC 6803 is the most widely studied model cyanobacterium, with a well-developed omics level knowledgebase. Like the lifestyles of other cyanobacteria, that of Synechocystis PCC 6803 is tuned to diurnal changes in light intensity. In this study, we analyzed the expression patterns of all of the genes of this cyanobacterium over two consecutive diurnal periods....