PAGE gel, individual proteins were either visualized by Coomassie Brilliant Blue stain excised and subjected to an in-gel tryptic digest (digests were performed as described (Shevchenko et al, 1996), or the eluted CenH3 particles were precipitated with 10% trichloroacetic acid, washed once with acetone, dried, re-dissolved in de-ionized water, and digested in solution with Arg-C protease. For C...

Chemical ionization mass spectrometry (MS) and tandem mass spectrometry (MS/MS) experiments have been performed for the structural characterization and isomeric differentiation of two series of C- and O-linked arylglycosides with potential antioxidant activity. Different amines have been used for producing gas phase chemical ionization. Depending on their proton affinity and steric hindrance, a...

Citation: Nixon RL and Ross ARS (2016) Evaluation of Immobilized Metal-Ion Affinity Chromatography and Electrospray Ionization Tandem Mass Spectrometry for Recovery and Identification of Copper(II)-Binding Ligands in Seawater Using the Model Ligand 8-Hydroxyquinoline. Front. Mar. Sci. 3:246. doi: 10.3389/fmars.2016.00246 Evaluation of Immobilized Metal-Ion Affinity Chromatography and Electrospr...

Acylcarnitine analysis using tandem mass spectrometry has become a powerful tool in the investigation of pediatric patients presenting with clinical signs and symptoms suggestive of fatty acid oxidation defects. These signs are diverse and include failure to thrive, feeding difficulties, and cardiomyopathy. Because the signs and symptoms are nonspecific, the identification of acylcarnitines cha...

Citation: Zhang X, Li J, Xie B, Wu B, Lei S, Yao Y, He M, Ouyang H, Feng Y, Xu W and Yang S (2018) Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry. Front. Pharmacol. 9:282. doi: 10.3389/fphar.2018.00282 Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Muci...

A metabolite of cyclosporine has been isolated and its structure identified through use of HPLC and tandem mass spectroscopy. Fast atom bombardment mass spectrometry of an HPLC fraction co-eluting with 1 eta hydroxy-cyclosporine (M17) indicated that the mass of this metabolite was 2 Da greater than that of cyclosporine. Further isolation by HPLC yielded a pure fraction, which we analyzed with t...

organic acidemias, also known as organic acidurias, are a group of disorders characterized by increased excretion of organic acids in urine. they result primarily from deficiencies of specific enzymes in the breakdown pathways of amino acids or from enzyme deficiencies in beta oxidation of fatty acids or carbohydrate metabolism. organic acids also are found in the urine of some patients with mi...

High-field asymmetric waveform ion mobility spectrometry (FAIMS) is shown to be capable of resolving isomeric and isobaric glycosaminoglycan negative ions and to have great utility for the analysis of this class of molecules when combined with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and tandem mass spectrometry. Electron detachment dissociation (EDD) and other ion...