Neuroimaging and in particular functional magnetic resonance imaging (fMRI) of olfactory function relies on the ability to model the time course of brain responses elicited by odor stimuli. In this study we compared two templates of olfactory brain activation by comparing levels of correlation in regions critical to olfactory processing with either a stimulation-based template or a perception-b...

Modified trinucleotides, 3MeU-A-G+, 3MeU-A-A, 5MeUA-G, 5MeU-A-A, BrU-A-G, hU-A-G, and U-A-I (see J. BioZ. Chem., 241, 527 (1966)), were synthesized by a combination of organic and enzymatic methods. The activity of each trinucleotide as a template for peptide chain termination was determined. The attachment of a methyl group to the NB position of uracil eliminates the template activity of termi...

The effect of chromatin proteins on template activity has been studied. Using both E. coli RNA polymerase and calf thymmus polymerase B we have measured the number of initiation sites on chromatin and various histone-DNA complexes. Chromatin can be reconstituted with histone proteins alone and this complex is still a restricted template for RNA synthesis. The removal of histone f1 causes a larg...

In biomechanics, explanatory template models are used to identify the basic mechanisms of human locomotion. However, model predictions often lack verification in a realistic environment. We present a method that uses template model mechanics as a blueprint for a bipedal robot and a corresponding computer simulation. The hypotheses derived from template model studies concerning the function of h...

OBJECTIVE The aims of this study were to determine the feasibility of introducing a computerized template for identifying quality of care during an OA consultation, describe quality of OA care in practices in which the template was introduced and assess the effect of the template on routinely recorded clinician behaviour in those practices. METHODS A computerized template to assist the record...

In vitro studies have indicated that retroviral nucleocapsid (NC) protein facilitates both DNA synthesis by reverse transcriptase (RT) and annealing of the nascent DNA with acceptor template. Increasing the rate of DNA synthesis is expected to reduce the frequency of RT template switching, whereas annealing the nascent DNA with acceptor template promotes template switching. We performed a mutat...

Specific polyanions release DNA template restrictions for DNA synthesis in isolated rat liver nuclei. The degree to which DNA synthesis is enhanced can be correlated with a spectrum of changes in nuclear structure Each polyanion which is effective in the release of template restriction produces a characteristic alteration in nuclear ultrastructure. Polyanions which have no effect on DNA synthes...

It is often assumed that a polymerase moves along the template as it synthesizes RNA. However, a polymerase that tracks along a helical strand will generate a transcript that is entwined about the template. No such interlocking results if the polymerase is immobile and the template moves past it. Therefore we investigated whether immobilization inhibits the RNA polymerase of T7 bacteriophage us...