Out of various industrial enzymes, thermostable βglucosidases are of great interest, due to their utility in conversion of lingocellulosic material into bioethanol. Determination of the factors responsible for thermostability in β-glucosidases by sequence and structure comparison will be useful in identification, isolation and design of novel thermostable βglucosidases. In the present study the...

Thermostability is one of the product characteristics preferred by WHO for vaccines against respiratory infections due to ease administration, pain minimization, and low costs. Thermostable dry powder inhaler (DPI) vaccine formulations can induce protective antibodies T cells at site infection in lungs. However, majority licensed human based on liquid dosage forms, there no mucosal adjuvants. T...

Using directed evolution and site-directed mutagenesis, we have isolated a highly thermostable variant of Aspergillus niger glucoamylase (GA), designated CR2-1. CR2-1 includes the previously described mutations Asn20Cys and Ala27Cys (forming a new disulfide bond), Ser30Pro, Thr62Ala, Ser119Pro, Gly137Ala, Thr290Ala, His391Tyr and Ser436Pro. In addition, CR2-1 includes several new putative therm...

D-Hydantoinase is currently employed as a biocatalyst for the production of optically pure D-amino acids, which are intermediates for the synthesis of semisynthetic antibiotics, peptide hormone, pyrethroids, and pesticides. In the process developed by the Yamada group,1,2 DL-5-substituted hydantoin is asymmetrically hydrolyzed to the N-carbamoyl-D-amino acid by D-specific hydantoinase, and this...

An effective AIDS vaccine will need to protect against globally diverse isolates of HIV. To address this issue in macaques, we administered a live-attenuated simian immunodeficiency virus (SIV) vaccine and challenged with a highly pathogenic heterologous isolate. Vaccinees reduced viral replication by approximately 2 logs between weeks 2-32 (P < or = 0.049) postchallenge. Remarkably, vaccinees ...

background: pectinases are pectin degrading class of enzymes including polygalacturonase (pg), polymethyl galacturonase (pmg), pectate lyase (pel), and pectin esterase (pe) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices.objectives: a highly methylated pectin degrading bacterium from soil covered with fruit was...

The neurotensin receptor NTSR1 binds the peptide agonist neurotensin (NTS) and signals preferentially via the Gq protein. Recently, Grisshammer and co-workers reported the crystal structure of a thermostable mutant NTSR1-GW5 with NTS bound. Understanding how the mutations thermostabilize the structure would allow efficient design of thermostable mutant GPCRs for protein purification, and subseq...