Yarrowia lipolytica as an oleaginous yeast is capable of growing in various non-conventional hydrophobic substrate types, especially industrial wastes. In this study, the content thiamine (vitamin B1), riboflavin B2), pyridoxine B6), biotin B7) and folic acid B9) wet biomass Y. strains cultivated biofuel waste (SK medium), compared to standard laboratory YPD medium, was assessed. Additionally, ...

Although antibodies and aptamers are commonly used bioaffinity recognition elements, they are not available for many important analytes. As an alternative, we demonstrate use of a periplasmic binding protein (PBP) to provide high affinity recognition for thiamine (vitamin B1), an analyte of great importance to human and environmental health for which, like so many other small molecules, no suit...

Aims: To assess the effect of mandatory thiamine enrichment of wheat flour on blood thiamine levels in an alcoholdependent population. Methods: Alcohol-dependent clients (n = 100) entering an inpatient service for the management of alcohol withdrawal had thiamine blood tests and diet interviews. Approximately half (n = 46) the alcohol-dependent participants reported taking vitamin supplements p...

The present data show a fast and efficient biological sample processing method for the extraction of thiamine (vitamin B1) and its mono-(TMP) and di(TDP) phosphate esters from hippocampus, thalamus and prefrontal cortex (PFC) and blood sample of the rodents. In addition, using the hippocampus and standards of these three compounds we validated an isocratic fluorescence HPLC procedure for a simu...

Hemolytic effect of some water-soluble vitamins (niacin B5, pyridoxine B6, thiamine B1 and ascorbic and acid C) on erythrocytes was studied spectrophotometrically at relatively high concentration. The oxidation mechanism of hemoglobin was the same for the used vitamins. Vitamin C was the strongest hemolytic agent in comparison with the other vitamins, while vitamin B1 is the weakest one. The re...

BACKGROUND Vitamin B1 (thiamine-diphosphate) and B6 (pyridoxal-5'phosphate) are micronutrients. Analysis of these micronutrients is important to diagnose potential deficiency which often occurs in elderly people due to malnutrition, in severe alcoholism and in gastrointestinal compromise due to bypass surgery or disease. Existing High Performance Liquid Chromatography (HPLC) based methods inclu...

In the present communication, a comparative HPLC analysis of water-soluble vitamins (B-group) was carried out in in vitro and ex vitro germinated chickpea (Cicer arietinum L.) seeds. Quantitative analysis of ex vitro and in vitro germinated seedlings showed significant differences in vitamin contents. Maximum amount with a linear increase in B1 (thiamine HCl), B2 (riboflavin), and B3 (nicotinam...