Razzell, W. E. (British Columbia Research Council, Vancouver, Canada) and P. C. Trussell. Isolation and properties of an iron-oxidizing Thiobacillus. J. Bacteriol. 85:595-603. 1963. - An organism isolated from acidic copper-leaching waters has been shown to oxidize ferrous ions, sulfur, and metallic sulfides but exhibit peculiar responses to thiosulfate. The name Thiobacillus ferrooxidans has b...

The rusticyanin gene from the acidophilic chemolithotroph Thiobacillus ferrooxidans has been cloned and sequenced. A central portion of the gene was identified by PCR reactions utilising primers optimised for codon bias followed by nested PCR with degenerate primers. The 5' and 3' ends of the rusticyanin gene were then cloned using degenerate primers to each end and anchor sequences to the know...

Comparative sequence analysis of in vitro-amplified 16S rRNA genes of Thiosphaera pantotropha GB17T (T = type strain) and Paracoccus denitrificans LMG 4218T revealed identical 16S rRNA primary structures for the two organisms. The level of overall DNA similarity of Thiosphaera pantotropha GB17T and P. denitrificans DSM 65T is 85%, as determined by quantitative DNA-DNA hybridization. Therefore, ...

RP1, a broad-host-range incompatibility group P1 plasmid specifying multiple drug resistances, has been transferred into the chemolithotrophic bacterium Thiobacillus neapolitanus. The ability of T. neapolitanus to receive, express, and transmit RP1-encoded antibiotic resistances was examined. The data show that this obligate chemolithotroph can accept, replicate, and express heterologous plasmi...

inoculation of phosphate-solubilizing microorganisms along with rock phosphate is known to enhance the available p from soil. the success of such solubilization is largely dependent on the ability of the inoculant strain to survive under adverse environmental conditions. in this context, liquid inoculants are gaining importance and are becoming popular with longer shelf-life. in the present inv...

the aim of the experiment was to determine the effect of different dry matter content and addition of silage inoculant on the ruminal protein degradability. alfalfa herbage was collected from two cuts and the fresh matter was sampled two times during each cut (with different dry matter content). during the preparation of experimental micro-silages, the chopped forage was treated with a microbia...

introduction: sulfur is the key element for higher crops and plays an important role in the formation of proteins, vitamins, and enzymes. it is a constituent of amino acids such as cysteine and methionine, which act for the synthesis of other compounds containing reduced sulfur, such as chlorophyll and utilization of phosphorus and otheressential nutrients.deficiency of this nutrient in soil is...

The synthesis, molecular structure, and electronic structure characterization of two five-coordinate high-spin imidazolate-ligated iron(II) porphyrinates are reported. Their electronic structure, as deduced from Mössbauer spectra obtained in strong magnetic fields, is distinctly different from that of the analogous imidazole-ligated species. The resulting electronic structure models are consist...