Advances in numerical methods and three-dimensional imaging techniques have enabled the quantification of cardiovascular mechanics in subject-specific anatomic and physiologic models. Patient-specific models are being used to guide cell culture and animal experiments and test hypotheses related to the role of biomechanical factors in vascular diseases. Furthermore, biomechanical models based on...

The classic paradigm for in vitro tissue engineering of bone involves the isolation and culture of donor osteoblasts or osteoprogenitor cells within three-dimensional (3D) scaffold biomaterials under conditions that support tissue growth and mineralized osteoid formation. Our studies focus on the development and utilization of new dynamic culture technologies to provide adequate nutrient flux w...

Malignant cells cultured in three-dimensional (3D) models have been found to be 17 phenotypically and biochemically different from their counterparts cultured conventionally. Since 18 most of these studies employed solid cancers, how 3D culture affects multiple myeloma (MM) cells 19 is not well understood. Here, we compared MM cells (U266 and RPMI8226) in a 3D culture model 20 with those in con...

Two-dimensional (2D) monolayer cultures are the standard in vitro model for cancer research. However, they fail to recapitulate the three-dimensional (3D) environment and quickly lose their function. In this study, we developed a new 3D multicellular heterospheroid tumor model in a collagen hydrogel culture system that more closely mimics the in vivo tumor microenvironment for anti-cancer drug ...

most of river engineering problems are complicated and need to be analyzed by three dimensional models of flow and sediment transport. considering the fact that computers and numerical methods with great ability of flow and sediment transport simulation are developing quickly, using numerical modeling by engineers is inevitable. nevertheless, before application, numerical models need to be veri...

We have recently shown that a combination of microRNAs, miR combo, can directly reprogram cardiac fibroblasts into functional cardiomyocytes in vitro and in vivo. Reprogramming of cardiac fibroblasts by miR combo in vivo is associated with improved cardiac function following myocardial infarction. However, the efficiency of direct reprogramming in vitro is relatively modest and new strategies b...