نتایج جستجو برای: throughput nucleotide sequencing
تعداد نتایج: 307622 فیلتر نتایج به سال:
this study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. the community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and illumina high-throughput sequencing, respectively. chemical oxygen demand eff...
This thesis is based on the following publications, which are referred to in the text by their Roman numerals. " Silhouette " scores for assessment of SNP genotype clusters. BMC Genom-ics.resolution, high-throughput SNP mapping in Drosophila melanogaster. Positional cloning by fast-track SNP-mapping in Droso-phila melanogaster. Dried reagents for multiplex genotyping by tag-array minisequencing...
Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm
Tumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level. To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell sequencing method using two lymphoblastoid cell line single cells. We then carried out whole-exome s...
Whole-genome DNA methylation sequencing provides both methylation patterns and genetic information. We utilized base resolution methylomes to directly identify allelic linkage of DNA methylation and genomic variants. The paired association was further extended to construct hepitypes by the simultaneous phasing of genotype and methylation. Using such approach, the sequencing reads provide direct...
Tobacco mosaic virus (TMV) is one of the devastating plant viruses in the world that infects more than 200 plant species. Movement protein plays a supportive role in the movement of other plant viruses, and viral coat protein is highly expressed in infected plants and affects replication and movements of TMV. In order to investigate genetic variation in the terminal fragment sequence in Iranian...
The advent of next-generation sequencing allows simultaneous processing of several genomic regions/individuals, increasing the availability and accuracy of whole-genome data. However, these new approaches may present some errors and bias due to alignment, genotype calling, and imputation methods. Despite these flaws, data obtained by next-generation sequencing can be valuable for population and...
The transcriptomes of bread wheat Yunong 201 and its ethyl methanesulfonate derivative Yunong 3114 were obtained by next-sequencing technology. Single nucleotide variants (SNVs) in the wheat strains were explored and compared. A total of 5907 and 6287 non-synonymous SNVs were acquired for Yunong 201 and 3114, respectively. A total of 4021 genes with SNVs were obtained. The genes that underwent ...
Background: Argas persicus has a great importance for health and veterinary, it can transmit many infectious agents such as Borrelia anserina (avian spirochetosis) and Aegyptianella pullorum. Distinguishing Argasidae due to close morphological relationship is difficult. OBJECTIVES: In the present study, we performed molecular analyses based on PCR and sequencing of Amplicon derived from 16S rRN...
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