conclusions evidence of t. gondii occurrence in sheep and camels was provided in the provinces under study. there is a need to investigate the potential risk factors of zoonotic infections. furthermore, animal health and production losses caused by the parasite; and associated zoonotic implications in the area under study need to be explored. materials and methods animals in the field and those...

background cholera is a severe diarrheal illness caused by vibrio cholerae, which produces a virulence factor named el tor hemolysin encoded by the hlya gene. objectives this study meant to analyze the phenotypic characteristics and presence of hlya gene in v. cholerae isolated from patients in iran. the hlya gene which codes for hemolysin, plays an essential role in manifestation of cholera ,a...

background brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including iran. diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. rapid detection of brucella species by an automated blood culture system and polymerase chain reaction (pcr) may l...

background: some dynamic changes occurs during spermatogenesis such as histone removal and its replacement with transition nuclear protein and protamine. these proteins are required for packing and condensation of sperm chromatin. jhdm2a is a histone demethylase that directly binds to promoter regions of tnp1 and prm1 genes and controls their expression by removing h3k9 at their promoters.objec...

background: human enteroviruses (evs) may have a role as a possible risk factor in the pathogenesis of mi. the aim of this study was to evaluate the presence of enterovirus genomic rna in peripheral blood samples of patients with acute myocardial infarction (mi). methods: we investigated the presence of enterovirus genomic rna in the peripheral blood of 115 patients with acute mi hospitalized i...

background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...

results no borrelia dna was detected by pcr in skin lesions of patients with morphea. patients and methods sixty six patients with morphea were prospectively included in the present study. for each patient, formalin-fixed, paraffin-embedded tissue blocks of skin lesion biopsies were examined for borrelia spp. dna using pcr. objectives we aimed to find the frequency of borrelia in morphea lesion...

conclusions this study suggests that the vaca pcr can be very useful in diagnosis, treatment, and follow-up of patients with gastritis and pud. the pcr assay and clo test can complement and confirm each other in identification and eradication of cancer-related strains of h. pylori. the exclusion of patients with suspected achlorhydria from the study is likely a reason for the lack of false posi...

streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. the aim of this study was to evaluate pcr assay compared with conventional culture method for direct detection of streptococcus agalactiae . total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from june 2013 through february 2014 for detection of streptococcus...

background :polymerase chain reaction (pcr) assay has widely used for the detection of tuberculosis (tb). this study tried to compare in-house pcr with some well-known commercial pcr kits for detection of tb agent. methods : clinical samples obtained from 620 tb suspected patients were analyzed for the diagnosis of mycobacterium tuberculosis complex (mtc) by in-house pcr. all samples were obtai...