The genotoxicity of the ethanolic extract betel nut was evaluated using sarcoma 180 tumour bearing mouse considering sperm motility, viability, biochemical estimation fructose in seminal fluid and head morphology assays. Sperm studied by H-E staining Toluidine blue method. But method is a reliable to evaluate DNA damage sperms. Ethanolic BNE (betel extract) can suppress percentage viability lev...

The patterns of distribution of histamine and norepinephrine among the 4 chambers of the heart of rats, guinea pigs, and rabbits and among 15 portions of the dog heart were quite similar, except for the coronary ring of the dog, which was disproportionately high in histamine. In whole mouse hearts the separate chambers were not assayed; in the whole heart, the contents of the 2 amines did not c...

Background: Cell-based treatment approach using differentiated mesenchymal stem cells (MSCs) and mature chondrocytes has been considered as an advanced treatment for cartilage repair. We investigated the differentiated level of these two cell types that is crucial for their repair capacity for cartilage defect at a co-culture micro mass system. Methods: Passaged-2 MSCs isolated from the mouse b...

Sodium butyrate induces basophilic differentiation of HI60 promyelocytic leukemia cells that have been previously passaged in alkaline medium. A factor present in Mo conditioned medium (Mo-CM) acts synergistically with sodium butyrate to promote basophilic maturation in a dose-dependent fashion. The induced HI-60 cells exhibit nuclei at various stages of maturity and cytoplasmic granules staini...

A simple procedure for selective staining of acidic nuclear proteins is described, in which toluidine blue 0 was employed at pH 9. The acidic proteins are present in nucleoli, the nuclear ribonucleoprotein network, the region of nuclear membrane, and the cytoplasm. They are found also in intimate association with chromosomes in mitotic figures, and form a thin peripheral layer around them. Hist...

A 20-year-old female Japanese macaque, weighing 8.7 kg, developed severe pulmonary acariasis. Numerous whitish nodules, 2-4 mm, were scattered throughout the lungs. Histologically multifocal granulomatous lesions consisting of a large number of eosinophils, epithelioid cells, foreign body type giant cells, and collagen fibers were aggregated around the mait bodies. Numerous mast cells were also...

Mast cells in the left cranial pulmonary lobe of colostrum-deprived neonatal calves were quantified 2 and 6 h after intrabronchial inoculation with Mannheimia (Pasteurella) haemolytica A1. The mast cells were detected (1) immunohistochemically with a mouse anti-human mast cell tryptase monoclonal antibody, and (2) by metachromatic staining with low pH toluidine blue. A greater number of mast ce...

BACKGROUND Early detection holds the key to an effective control of cancers in general and of oral cancers in particular. However, screening procedures for oral cancer are not straightforward due to procedural requirements as well as feasibility issues, especially in resource-limited countries. METHODS We conducted a cross-sectional study to compare the performance of chemiluminescence, tolui...

Objective: The incidence of oral cancer worldwide varies 2-18% and in India 0.1 to 13.5%. Early evaluation of oral precancerous lesions can have dramatic effect on oral cancer mortality rate. Among the premalignant stage, leukoplakia is the most commonly encountered clinical lesion and epithelial dysplasia is most important predictive factor. The objective of this paper is to compare the effica...