Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans. Several infections caused by flatworms are considered major neglected diseases affecting countries in the America...

objective: electroporation can be a highly efficient method for introducing the foreign genetic materials into the targeted cells for transient and/or permanent genetic modification. considering the application of this technique as a very efficient method for drug, oligonucleotide, antibody and plasmid delivery for clinical applications and production of transgenic animals, the present study ai...

RALA is a cationic amphipathic peptide which has shown great promise as an efficient, multifunctional delivery system for the of nucleic acids. Rational design was utilised in this study to understand essential amino acids required and if any improvements could be made. Six peptides were synthesised with strategic sequences acid substitutions reduce sequence, while maintaining functional charac...

Objective: To investigate the expression of miR-642 in gastric cancer and its mechanism proliferation migration. Methods: qRT-PCR was used to detect cancer. Dual luciferase gene reporting system verify direct regulatory effect on KRT19 3' UTR. The mRNA protein levels after transfection were detected by Western blot. IncucyteS long term dynamic cell observation. Kaplan-Meier method analyze relat...

Adeno-associated viruses (AAV) are established vectors for delivering gene-therapy payloads, however the cost of goods transfection reagents and DNA, along with challenges to scaling-up process, presents obstacles industrialization recombinant adeno-associated virus (rAAV) therapeutic products overcome. The ELEVECTA® platform from CEVEC is a stable cell line inducible AAV production w...

objective(s): carbon nanotube (cnt) has been widely applied at molecular and cellular levels due to its exceptional properties. studies based on conjugation of cnts with biological molecules indicated that biological activity is preserved. polyethylenimine (pei) is explored in designing novel gene delivery vectors due to its ability to condense plasmid dna through electrostatic attraction. in t...

background: the poor permeability of the plasma and nuclear membranes to dna plasmids are two major barriers for the development of these therapeutic molecules. therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. objectives: the aim of this study was to investigate the in vitro delivery of plasmid dna encoding hpv16 e7 gene ...

Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for theimprovement of low transfection efficiency of chitosan. The present study aims to test thepulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposedto aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg-PEI complexes for 30 min during the development of ...

This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DC...