Portulaca oleracea L. is a medicinal plant find in Europe and Asia. This plant contains valuable secondary metabolite such as noradrenalin. Naturally the wild plant produce small amount of this secondary metabolites so induction of hairy roots can be useful to increase its production. In our research Agrobacterium rhizogenes strain AR15834 was used to induce hairy roots in the two-week leaf exp...

A cDNA clone that encodes a feedback-insensitive anthranilate synthase (AS), ASA2, isolated from a 5-methyl-tryptophan (Trp) (5MT)-resistant tobacco cell line under the control of the constitutive cauliflower mosaic virus 35S promoter, was introduced into the forage legume Astragalus sinicus by Agrobacterium rhizogenes with kanamycin selection. The 35S-ASA2 gene was expressed constitutively as ...

Salvia miltiorrhiza is an annual plant growing in China, Mongolia, Korea and some other Asian countries. The extract from S. miltiorrhiza roots has been used for supporting healthy cardiovascular and circulatory systems during the last decade. The active constituents of S. miltiorrhiza from different areas vary significantly, and the wild resources are overexploited. To adapt the demand for act...

Background: Trigonella foenum-graecum L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. Objective: In this study, hairy roots culture as a novel method for trigonelline production was evaluated. Methods: For optimizing the hairy roots culture of Trigonella foenum-graecum, three strains of Agrobacterium rhyzogenes (ATCC15834, MSU440 and K...

Pigmented naphthoquinone derivatives of shikonin are produced at specific times and in specific cells of Lithospermum erythrorhizon roots. Normal pigment development is limited to root hairs and root border cells in hairy roots grown on "noninducing" medium, whereas induction of additional pigment production by abiotic (CuSO4) or biotic (fungal elicitor) factors increases the amount of total pi...

Seeds of Silybum marianum which have been used to treat liver diseases have an active component called silymarin. Hairy root cultures of S. marianum could be able to produce silymarin. There is evidence that the use of elicitors has been an important strategy for improving the production of secondary metabolites. Elicitation is an interesting topic to investigate cell signaling pathway in secon...

the aim of this study was to evaluate and compare the sensitivity of plant bioassays:tradescantia-micronucleus (trad-mcn) tests using bnl 02 and 4430 clones, and crepis capillaris hairyroots chromosome aberrations test. the evaluation of the sensitivity of the tests was based on the example of the analysis of the genotoxicity of water samples from two natural water reservoirs in poland: the raw...

A method named "bag culture" was developed for coculturing of Linum persicum (section Syllinum) and L. austriacum (section Linum) hairy roots. For this propose L. austriacum and L. persicum hairy root cultures were established using Agrobacterium rhizogenes in McCown medium. L. persicum hairy roots in bags (1 mm2 mesh) were successfully grown together with L. austriacum hairy roots. The amounts...

Stable transformation of Mesembryanthemum crystallinum L. (common ice plant) with a green fluorescent protein (GFP) construct targeted to the endoplasmic reticulum was obtained. Seven and fourteen days after germination seedlings were infected with Agrobacterium rhizogenes strain ARqua1 either by direct coating of the cut radicles with bacteria growing on solid medium or by immersion of the cut...