We have used the Koppes and Nordstrøm (Cell 44:117-124, 1986) CsCl density transfer approach for analysis of DNA from exponentially growing, isogenic Escherichia coli dam+ and dam mutant cells to show that timing between DNA replication initiation events is precise in the dam+ cells but is essentially random in the dam cells. Thus, methylation of one or more GATC sites, such as those found in u...

This paper reports on a study of the temporal characteristics of phonological error repair in spontaneous Dutch speech, with a focus on how the articulation rate of the correct target word production — the repair — compares to that of the preceding erroneous target word attempt — the reparandum. The study is motivated by two findings from recent independent studies: first, that selfrepair is ge...

Practicing with the use of a ball machine could handicap a player compared to playing against an actual opponent. Recent studies have shown some differences in swing timing and movement coordination, when a player faces a ball projection machine as opposed to a human opponent. We focused on the time of movement initiation and on stroke timing during returning tennis serves (simulated by a ball ...

BACKGROUND The safety and timing of venous thromboembolism (VTE) prophylaxis in patients with blunt splenic injuries is not well known. We hypothesized that early initiation of VTE prophylaxis does not increase failure of nonoperative management or transfusion requirements in these patients. METHODS A retrospective review of trauma patients with blunt splenic injury was performed. Patients we...

In eukaryotes, duplication of genomic information depends on the sequential activation of multiple replication origins distributed along the chromosomes. Replication origins differ in initiation time, chromatin structure and three-dimensional position in the nucleus. Recently, we have performed a systematic analysis of the role of histone deacetylases (HDACs) in the regulation of origin activit...

OBJECTIVES This study evaluated timing of β-blocker initiation before surgery and its relationship with: 1) pre-operative heart rate and high-sensitivity C-reactive-protein (hs-CRP) levels; and 2) post-operative outcome. BACKGROUND Perioperative guidelines recommend β-blocker initiation days to weeks before surgery, on the basis of expert opinions. METHODS In 940 vascular surgery patients, ...

Materials and methods. This study analyzed the results of treatment 62 patients with severe COVID-19 in intensive care unit using selective hemoadsorption cytokines. All were admitted to within 14 days from disease onset subdivided into two groups. Group 1 ( n =32) received on a top standard hemoperfusion (HP) procedure for 4 hours, 2–3 row, cytokine sorption column composed mesoporous styrene-...

BACKGROUND There is growing evidence that supports the benefits of early use of caffeine in preterm neonates with RDS; however, no formal recommendations specifying the exact timing of therapy initiation have been provided. OBJECTIVES We compared neonatal outcomes in infants receiving early (initial dose on the 1st day of life) and late (initial dose on day 2+ of life) caffeine therapy. MET...

Initiation stage of in vitro culture grapes is a critical clonal micropropagation, the purpose which to obtain maximum number sterile and viable regenerative plants.
 Modern varieties are hybrids between main species Vitis vinifera, V. labrusca, riparia, amurensis, rupestris,
 berlandieri lincecumii, require selection optimal cultivation conditions: timing initiation,
 concentrat...

Quality of life is impaired in patients with epilepsy and can be improved by effective therapy. Randomised clinical trials have shown that lamotrigine treatment is associated with improved quality of life. However, little information is available on quality of life or treatment effects in patients with epilepsy in the general population. The objective of this study was to estimate the impact of...