The U4/U6.U5 triple small nuclear ribonucleoprotein (tri-snRNP) is a major spliceosome building block. We obtained a three-dimensional structure of the 1.8-megadalton human tri-snRNP at a resolution of 7 angstroms using single-particle cryo-electron microscopy (cryo-EM). We fit all known high-resolution structures of tri-snRNP components into the EM density map and validated them by protein cro...

A highly concise and effective synthesis of the mannose octasaccharide of the N-linked glycan in the adhesion domain of human CD2 was achieved via TMSOTf-promoted selective 6-glycosylation of a trisaccharide 4,6-diol acceptor with a pentasaccharide donor, followed by deprotection. The pentasaccharide was constructed by selective 3,6-diglycosylation of 1,2-O-ethylidene-beta-D-mannopyranose with ...

The Toxics Release Inventory (TRI) requires facilities with 10 or more full-time employees that process > 25,000 pounds in aggregate or use > 10,000 pounds of any one TRI chemical to report releases annually. However, little is known about releases from non-TRI-reporting facilities, nor has attention been given to the very localized equity impacts associated with air toxics releases. Using geog...

This paper presents a new tri-dexel volumetric modeling method for representing complex geometric models in applications such as haptic virtual sculpting and product prototyping. A tri-dexel model consists of three overlapping dexel models in X, Y, and Z axis directions, respectively. Detailed algorithms of eliminating inconsistency in a tri-dexel model and reconstructing a water-tight polyhedr...

In this work, we present TRI-DIRECT, an interactive visual analytic system with capabilities to analyze spatio-temporal data for both professional and novice users. The system is motivated by the Toxic Release Inventory (TRI) program of US Environmental Protection Agency (EPA) and its associated datasets; the TRI program was created in 1986 to track toxic chemical usage, which includes release,...

BACKGROUND There is growing evidence that transradial (TRI) as compared to transfemoral (TFI) percutaneous coronary intervention (PCI) is associated with improved clinical outcome driven by less hemorrhagic complications, in particular in STEMI patients receiving aggressive antithrombotic treatment. Feasibility rate of TRI in STEMI patients has not yet been evaluated. METHODS/RESULTS Four-hun...

BACKGROUND AND PURPOSE Treatment with triglyceride emulsions of docosahexaenoic acid (tri-DHA) protected neonatal mice against hypoxia-ischemia (HI) brain injury. The mechanism of this neuroprotection remains unclear. We hypothesized that administration of tri-DHA enriches HI-brains with DHA/DHA metabolites. This reduces Ca2+-induced mitochondrial membrane permeabilization and attenuates brain ...

R epo contracts are a kind of collateralized loan that has become predominant in the United States among large cash investors. There are several types of repo contracts, such as bilateral delivery-versuspayment repos, interdealer repos, and tri-party repos. A significant portion of repo transactions in the United States take the form of tri-party repos, where a third party (a clearing bank) pro...

The U4/U6.U5 tri-snRNP is a key component of spliceosomes. By using chemical reagents and RNases, we performed the first extensive experimental analysis of the structure and accessibility of U4 and U6 snRNAs in tri-snRNPs. These were purified from HeLa cell nuclear extract and Saccharomyces cerevisiae cellular extract. U5 accessibility was also investigated. For both species, data demonstrate t...