This research evaluated the impact of emulsion interfacial composition on in vitro small intestinal lipolysis kinetics with inclusion rabbit gastric lipase resulting a prelipolysis step. O/w emulsions contained 5% triolein (w/w) and 1% following emulsifiers: sodium taurodeoxycholate, citrus pectin, soy protein isolate, lecithin, tween 80. Emulsions were subjected to static digestion diverse spe...

During recent years there has been increasing interest in the study of fat absorption, particularly in the investigation of conditions associated with steatorrhoea. The need for a single diagnostic test for steatorrhoea, suitable for use with both outpatients and in-patients, has become apparent. Ideally, such a test should be easy to carry out for the patient and nursing staff as well as for t...

were calculated as eight hour cumulative values and expressed as percentages of the administered dose. Reference ranges have been calculated separately for the following age groups: 65 years or less (201 to 460), 66 to 75 (182 to 405) and over 75 (141 to 336). A clear age related decline in eight hour cumulative values was noted. The values fell by 23% between the ages of 17 and 65 years and by...

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored ...

Prediction and preliminary modelling of equilibrium data represents the best tool for obtaining the real picture of reaction system when the experimental data are not available or can not be easily measured. The complex reaction mixture under supercritical condition is one such system and for its modelling two main problems usually exists. The first is the approximation of real data at critical...