A membrane filtration method was examined concerning the effective collection of iron(III) from a homogeneous aqueous solution with Chromazurol B (CAB), one of the triphenylmethane dyes, as a precipitating reagent in the presence of a non-ionic surfactant, polyethylene glycol mono[4-(1,1,3,3-tetramethylbutyl)phenyl]ether (Triton X-100). A formed blue Fe(III)-CAB complex was collected as a preci...

UNLABELLED The canalicular plasma membrane is constantly exposed to bile acids acting as detergents. Bile acids are essential to mediate release of biliary lipids from the canalicular membrane. Membrane microdomains (previously called lipid rafts) are biochemically defined by their resistance to detergent solubilization at cold temperature. We aimed to investigate the canalicular plasma membran...

The hypolipidemic activity of Lepidium sativum (family: Brassicaceae) seed sapogenin and flavonoid extract (LSTS and LSTF) was studied against Triton x-100 and high cholesterol diet (HCD) induced hyperlipidemia on rats. The parameters assessed were serum total protein, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDLc), low density lipoprotein cholesterol (LD...

The hyaluronan receptor CD44 is an abundant glycoprotein expressed on a variety of different cell types. In fibroblasts a significant portion of receptor molecules remain in the detergent-insoluble fraction after Triton X-100 extraction. Detergent insolubility of these CD44 molecules has been interpreted to reflect their association with the cytoskeleton. In this study we examined the structura...

UDP-galactose-A-acetylglucosamine galactosyltransferase of rat liver Golgi apparatus is membrane-bound, that is, it is not released by treatment with salt or disruption of the Golgi saccules using N, decompression in a Parr bomb. The enzyme can be solubilized to greater than 90% by treatment of rat liver Golgi membranes with 2 mg of Triton X-lOO/ml. We have determined the sedimentation constant...

A protein fraction was obtained from human erythrocyte ghosts by solubilization with Triton X-100 or octylglucoside. Triton X-100 was removed from the protein by Bio-Beads SM-2 and octylglucoside, by diafiltration. The solubilized protein fraction catalyzed D-glucose uptake when reconstituted in sonicated liposomes. The uptake was time dependent and inhibited by mercuric ions or cytochalasin B....

General Experimental Procedures: Egg phosphatidylcholine (ePC) and routine chemicals were obtained from Sigma Chemical Co. SM-2 Bio-beads were purchased from Bio-Rad Laboratories. ULTROL grade Triton X-100 was from Roche Molecular Biochemicals. 1-acyl-2-C6-NBD-PC (NBD-PC) was from Avanti Polar Lipids. Salt-washed endoplasmic reticulum (SWER) was prepared from a rat liver homogenate as previousl...

Photosystem II reaction centers comprised of the Dl, D2 and cytochrome b-559 polypeptides were isolated from welldefined oxygen-evolving reaction center preparations using either a combination of LiClO, and dodecyhnaltoside, or Triton X-100 alone. Yields of chlorophyll and cytochrome b-559 for both preparative methods were compared, and pigment contents were compared based on 2.0 b-559 per reac...