background: plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. objective: plant root and shoot hyoscyamine (hyo) and scopolamine (sco) variations were investigated under three water deficit stress (wds) levels as 30 (w1), 60 (w2) and 90 % (w3) water depletion of field capacity an...

Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...

Berberine, a natural isoquinoline alkaloid of many medicinal herbs, has an active function against a variety of microbial infections including Microsporum canis (M. canis). However, the underlying mechanisms are poorly understood. To study the effect of berberine chloride on M. canis infection, a Digital Gene Expression (DGE) tag profiling was constructed and a transcriptome analysis of the M. ...

Cell suspension of Hyoscyamus a/bus L. were investigated for growth and production of tropane alkaloids in three different media. The effects of sucrose concentration and initial pH were also studied. 2,4Dichlorophenoxyacetic acid and 1% sucrose enhanced the production of tropane alkaloids. The percent of alkaloids (0.75%) was higher than in the cultivated plant (0.352 %).

Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...

Abstract The incorporation of counterions into a variety substances to control number their physiochemical properties is vital within range fields. Electrochemiluminescence (ECL) based sensors have grown in popularity recent years with employment observed across analytical and bioanalytical applications. ECL typically concerned the core electroactive functionality species interest, structural s...

The title compound, C(17)H(24)NO(5)S(+)·C(18)H(13)O(8) (-), is the key inter-mediate during the preparation of lesatropane [systematic name (1R,3S,5R,6S)-6-acetoxy-3-(4-methylphenylsulfonyloxy)tropane], a potential anti-glaucoma agent. The tertiary N atom of the tropane ring is involved in inter-molecular N-H⋯O hydrogen bonding, and the carboxylate groups are involved in inter-molecular O-H⋯O h...

opium poppy (papaver somniferum l.) has two major products: alkaloids in the capsules and the seeds. it is known that opium poppy today contains alkaloid in great quantities. opiate alkaloids and their synthetic derivatives are widely used in medicines which are produced in hundreds of tons for the medicine industry. the study aimed to screen the capsules of six turkish opium poppy lines to eva...