NMR titration experiments are a rich source of structural, mechanistic, thermodynamic and kinetic information on biomolecular interactions, which can be extracted through the quantitative analysis of resonance lineshapes. However, applications of such analyses are frequently limited by peak overlap inherent to complex biomolecular systems. Moreover, systematic errors may arise due to the analys...

This paper describes a database of 11 dependency treebanks which were unified by means of a two-dimensional graph format. The format was evaluated with respect to storage-complexity on the one hand, and efficiency of data access on the other hand. An example of how the treebanks can be integrated within a unique interface is given by means of the DTDB interface.

Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protei...

This review article revisits and outlines the perfectly matched layer (PML) method its various formulations developed over past 25 years for numerical modeling simulation of wave propagation in unbounded media. Based on concept complex coordinate stretching, an efficient mixed displacement-strain unsplit-field PML formulation second-order (displacementbased) linear elastodynamic equations is th...

The use of perfectly matched layers (PML) has recently been introduced by Berenger as a material absorbing boundary condition (ABC) for electromagnetic waves. In this paper, we will rst prove that a ctitious elastodynamic material half-space exists that will absorb an incident wave for all angles and all frequencies. Moreover, the wave is attenuative in the second half-space. As a consequence, ...

Soluble cytoplasmic extracts of Candida albicans groups A and B were prepared and compared by quantitative immunoelectrophoresis experiments performed with a commercial anti-C. albicans group A immune serum. Although crossed immunoelectrophoresis, tandem crossed immunoelectrophoresis, and line immunoelectrophoresis revealed many cross-reactions between the two groups, some components seemed to ...

Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.