نتایج جستجو برای: urease gene urec

تعداد نتایج: 1144991  

Journal: :Journal of bacteriology 2003
Stéphane Benoit Robert J Maier

The Helicobacter pylori ureE gene product was previously shown to be required for urease expression, but its characteristics and role have not been determined. The UreE protein has now been overexpressed in Escherichia coli, purified, and characterized, and three altered versions were expressed to address a nickel-sequestering role of UreE. Purified UreE formed a dimer in solution and was capab...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شاهد - دانشکده علوم پایه 1390

شترسانان در سرمشان نوع خاصی از ایزوتیپ igg را دارا می باشند که به علت نداشتن زنجیره سبک، آنتی بادی زنجیره سنگین hcab نام گذاری شده اند. بخش متغیر این نوع آنتی بادی(vhh)، دارای ویژگی های منحصر به فردی است که منجر به کاربرد وسیع آن در بیوتکنولوژی شده است. در این مطالعه هدف انتخاب vhh با اختصاصیت بسیار بالا علیه زیرواحد urec آنزیم اوره آز هلیکوباکترپیلوری می باشد. هلیکو باکتر پیلوری یک باکتری گرم ...

Journal: :The Journal of clinical investigation 2015
Ting-Chin David Shen Lindsey Albenberg Kyle Bittinger Christel Chehoud Ying-Yu Chen Colleen A Judge Lillian Chau Josephine Ni Michael Sheng Andrew Lin Benjamin J Wilkins Elizabeth L Buza James D Lewis Yevgeny Daikhin Ilana Nissim Marc Yudkoff Frederic D Bushman Gary D Wu

Increasing evidence indicates that the gut microbiota can be altered to ameliorate or prevent disease states, and engineering the gut microbiota to therapeutically modulate host metabolism is an emerging goal of microbiome research. In the intestine, bacterial urease converts host-derived urea to ammonia and carbon dioxide, contributing to hyperammonemia-associated neurotoxicity and encephalopa...

Journal: :Infection and immunity 2003
Jonathan D Dattelbaum C Virginia Lockatell David E Johnson Harry L T Mobley

Proteus mirabilis, a cause of complicated urinary tract infection, produces urease, an essential virulence factor for this species. UreR, a member of the AraC/XylS family of transcriptional regulators, positively activates expression of the ure gene cluster in the presence of urea. To specifically evaluate the contribution of UreR to urease activity and virulence in the urinary tract, a ureR mu...

Journal: :Journal of bacteriology 1999
D J McGee F J Radcliff G L Mendz R L Ferrero H L Mobley

Arginase of the Helicobacter pylori urea cycle hydrolyzes L-arginine to L-ornithine and urea. H. pylori urease hydrolyzes urea to carbon dioxide and ammonium, which neutralizes acid. Both enzymes are involved in H. pylori nitrogen metabolism. The roles of arginase in the physiology of H. pylori were investigated in vitro and in vivo, since arginase in H. pylori is metabolically upstream of urea...

Journal: :archives of clinical infectious diseases 0
mahrokhsar fotouhi islamic azad university, tonekabon branch, tonekabon, ir iran gita eslami department of microbiology, faculty of medicine, shahid beheshti university of medical sciences, tehran, ir iran; email: habibolah peirovi nanomedicine and tissue engineering research center, shahid beheshti university of medical sciences, tehran, ir iran mohammad hashemibahramani department of pathology, shahid beheshti university of medical sciences, tehran, ir iran masoumeh douraghi department of microbiology, faculty of medicine, shahid beheshti university of medical sciences, tehran, ir iran elahe ahmadzadeh nanomedicine and tissue engineering research center, shahid beheshti university of medical sciences, tehran, ir iran

prior investigators demonstrated helicobacter pylori as a risk factor of liver diseases. in this study association between h. pylori and hepatocellular carcinoma was investigated. patients and methods totally, 59 specimens of liver were collected from two health care centers affiliated to shahid beheshti university of medical sciences, including 22 specimens of hepatocellular carcinoma, 18 spec...

Journal: :Defence life science journal 2022

The current study was designed to isolate and characterize urease-producing bacteria assess their ability precipitate calcium carbonate. Total eight were isolated from dung-rich soil samples collected Dakor, Gujarat. Out of these, two bacterial strains designated as DGDK-3 DGDK-4 found produce a considerable level urease in the initial screening on urea agar medium. Based morphological physiolo...

2011
Stéphane L. Benoit Robert J. Maier

UNLABELLED A novel mechanism aimed at controlling urease expression in Helicobacter pylori in the presence of ample nickel is described. Higher urease activities were observed in an hp0868 mutant (than in the wild type) in cells supplemented with nickel, suggesting that the HP0868 protein (herein named Mua for modulator of urease activity) represses urease activity when nickel concentrations ar...

Journal: :Infection and immunity 1995
T F De Koning-Ward R M Robins-Browne

The stomach serves as a barrier to enteric infection because of the antibacterial effect of the hydrochloric acid in gastric juice. In this study, we tested the ability of the enteric pathogen Yersinia enterocolitica to tolerate a pH range of 2.0 to 6.0 and found that under the conditions of a normal human fasting stomach (pH < 3 and a gastric emptying time of 2 h), Y. enterocolitica is highly ...

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