Exonuclease VII was first identified in 1974 as a DNA exonuclease that did not require any divalent cations for activity. Indeed, Escherichia coli ExoVII was identified in partially purified extracts in the presence of EDTA. ExoVII is comprised of two subunits (XseA and XseB) that are highly conserved and present in most sequenced prokaryotic genomes, but are not seen in eukaryotes. To better u...

Occurrence of the hsp70 (dnaK) gene was investigated in various members of the domain Archaea comprising both euryarchaeotes and crenarchaeotes and in the hyperthermophilic bacteria Aquifex pyrophilus and Thermotoga maritima representing the deepest offshoots in phylogenetic trees of bacterial 16S rRNA sequences. The gene was not detected in 8 of 10 archaea examined but was found in A. pyrophil...

The alpha-L-fucosidase from Thermotoga maritima (Tm alpha fuc) was converted into alpha-L-transfucosidase variants by directed evolution. The wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a pNP-fucoside donor to pNP-fucoside (self-condensation) with alpha-(1-->3) regioselectivity or pNP-galactoside (transglycosylation) with alpha-(1-->2) regioselecti...

Salinity is a major abiotic stress that affects plant growth and productivity. To cope with salt stress, plants express large number of responsive genes proteins are involved in wide range cellular functions. In the present study, halophytic Suaeda maritima (L.) Dumort. were hydroponically exposed to NaCl for understanding molecular mechanisms behind salinity tolerance using PCR-based Suppressi...

The classical Baker-Campbell-Hausdorff formula gives a recursive way to compute the Hausdorff series H = ln(ee ) for noncommuting X, Y . Formally H lives in the graded completion of the free Lie algebra L generated by X, Y . We present a closed explicit formula for H = ln(ee ) in a linear basis of the graded completion of the free metabelian Lie algebra L̄ = L/[[L, L], [L, L]].

Cryopreservation of germplasm in liquid nitrogen (LN) is a perfect method for the long-term conservation of plant genetic resources. Lily [Lilium ledebourii (Baker) Bioss.], Persian lilac (Melia azedarach L.), and tea (Camellia sinensis L.) were evaluated for long-term storage in liquid nitrogen (LN) at -196oC. Encapsulation within alginate beads was shown to be beneficial in all studied specie...

background and objectives:  squill [drimia maritima (l.) stearn] is an important medicinal plant that has been used for medicinal purposes such as cardiovascular diseases and asthma since ancient times. bufadienolides are the main compounds of this plant and are responsible for some reported adverse effects. in order to reduce adverse effects, different methods like boiling with vinegar were ap...

Cultivated beets, including leaf garden fodder and sugar which belong to the species Beta vulgaris L., are economically important edible crops that have been originated from a halophytic wild ancestor, maritima L. (sea beet or beet). Salt drought major abiotic stresses, limit crop growth production most studied in beets compared other environmental stresses. Characteristically, salt- drought-to...