BACKGROUND Urinary albumin excretion is a sensitive diagnostic and prognostic marker for renal disease. Therefore, measurement of urinary albumin must be accurate and precise. We have developed a method to quantify intact urinary albumin with a low limit of quantification (LOQ). METHODS We constructed an external calibration curve using purified human serum albumin (HSA) added to a charcoal-s...

Hypoalbuminemic patients often have sufficient fluid accumulation to mandate diuretic therapy but are often resistant to diuresis. Studies have suggested that hypoalbuminemia itself impairs delivery of effective amounts of diuretic agent into the urine, the site of action. Therefore, administration of mixtures of albumin and loop diuretics may enhance responses. Thirteen patients with biopsy-pr...

OBJECTIVE Correlation of the urinary albumin excretion rate and the risk of death among elderly subjects. DESIGN 216 Subjects aged 60-74 whose urinary albumin excretion rate had been determined were followed up 62-83 months later. SETTING Municipality of Fredericia, Denmark. SUBJECTS 223 People who had been selected as control subjects for diabetics found during a systematic screening for...

Mini-slab electrophoresis on sodium dodecyl sulfate/polyacrylamide gel can be used to detect protein in unconcentrated urines containing more than 100 mg of protein per liter. Several urinary proteins--including albumin, transferrin, and the Tamm-Horsfall mucoprotein--precipitate, partly or totally, if urine samples are stored at 4 degrees C for one or two days. The present procedure permits id...

BACKGROUND We aimed to evaluate urinary MCP-1 and oxidative stress through urinary malondialdehyde (MDA) in leprosy and correlate them with traditional, but less sensitive markers of renal disease. METHODS This is a cross-sectional study of 44 patients with diagnosis of leprosy and no previous treatment. Skin smear was assessed through a bacteriological index - from 0 to 6+. Glomerular filtra...

Sir, Recently, our group conducted a supplementary study on urinary abnormalities following karate (kumite) competitions in professional female athletes. In a previous issue of NDT Plus [1], our group presented data showing significantly increased proteinuria of a mixed type (albumin and beta2-microglobulin or B2M) with prominence of albumin, as a glomerular urine protein, in 18 professional (1...

Sir, Recently, our group conducted a supplementary study on urinary abnormalities following karate (kumite) competitions in professional female athletes. In a previous issue of NDT Plus [1], our group presented data showing significantly increased proteinuria of a mixed type (albumin and beta2-microglobulin or B2M) with prominence of albumin, as a glomerular urine protein, in 18 professional (1...

BACKGROUND AND OBJECTIVES Albuminuria is a risk factor for cardiovascular and renal disease. However, little is known about the association of 24 h urinary sodium and potassium excretion with albuminuria in China. The aim of this study was to examine this association by analyzing the data from 1,975 Chinese adults living in north China. METHODS AND STUDY DESIGN Excretion of urinary sodium, po...

OBJECTIVE To investigate the relationship between early-onset retinopathy and urinary markers of renal dysfunction. RESEARCH DESIGN AND METHODS The Diabetes Incidence Study in Sweden (DISS) aims to register all new cases of diabetes in young adults (15-34 years). In 1987-1988, 806 patients were reported and later invited to participate in a follow-up study focusing on microvascular complicati...

• Potential living donors should have their urinary protein excretion measured using either a 24-hour urine collection (daily excretion) or a spot urine sample (protein/creatinine ratio). • A urine protein excretion of >300 mg/day (24 hour collection) or of >30 mg/mmol (spot urine protein/ creatinine ratio) is usually a contraindication to live donation. • Further investigations are warranted w...