Chitosan is a naturally occurring polymer derived from the deacetylation of chitin, which an abundant carbohydrate found mainly in shells various marine and terrestrial (micro)organisms. has been extensively used to construct nanoparticles (NPs), are biocompatible, biodegradable, non-toxic, easy prepare, can function as effective drug delivery systems. Moreover, chitosan NPs have employed gene ...

Transcription activator-like (TAL) effector nucleases (TALENs) have enabled the introduction of targeted genetic alterations into a broad range of cell lines and organisms. These customizable nucleases are comprised of programmable sequence-specific DNA-binding modules derived from TAL effector proteins fused to the non-specific FokI cleavage domain. Delivery of these nucleases into cells has p...

With an increased prevalence and understanding of central nervous system (CNS) injuries and neurological disorders, nucleic acid therapies are gaining promise as a way to regenerate lost neurons or halt disease progression. While more viral vectors have been used clinically as tools for gene delivery, non-viral vectors are gaining interest due to lower safety concerns and the ability to deliver...

Gene therapy and treatment with siRNA hold potential to treat a wide variety of different diseases. Genetic material is not usually stable and is generally hydrolyzed following intravascular administration. This makes the delivery of genes somewhat problematic since intact genetic material must usually be delivered intracellularly for therapeutic effect. For gene therapy, in most cases the gene...

viral protein-1 (vp1) is a major capsid protein of coxsakievirus b3 (cvb3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems vp1 can be considered as a vaccine candidate against cvb3 infection. in this study, cdna of vp1 was prepared, cloned into pet expression vector and...

During the 15 years since the discovery of type III human interferons [IFN-λ1(IL-29), IFN-λ2(IL-28A), and IFN-λ3(IL-28B)], numerous biological properties such as anticancer, antiviral, and immunomodulatory activities of this new IFN family have been investigated. Several studies have shown that the encapsulation of pcDNA with PLGA nanoparticles (NPs) protects them against DNase enzyme action an...

Gene therapy involves the introduction of foreign genetic material into cells in order exert a therapeutic effect. The application of gene therapy to the field of orthopaedic tissue engineering is extremely promising as the controlled release of therapeutic proteins such as bone morphogenetic proteins have been shown to stimulate bone repair. However, there are a number of drawbacks associated ...

Gene therapy involves the introduction of foreign genetic material into cells in order exert a therapeutic effect. The application of gene therapy to the field of orthopaedic tissue engineering is extremely promising as the controlled release of therapeutic proteins such as bone morphogenetic proteins have been shown to stimulate bone repair. However, there are a number of drawbacks associated ...

objective(s): although viral vectors are considered efficient gene transfer agents, their board application has been limited by toxicity, immunogenicity, mutagenicity and small gene carrying capacity. non-viral vectors are safe but they suffer from low transfection efficiency. in the present study, polyallylamine (paa) in two molecular weights (15 and 65 kda) was modified by alkane derivatives ...

Several improvements have been made in liposomal delivery, thus making this technology potentially useful for treatment of certain diseases in the clinic. Success in non-viral delivery is complicated and requires optimization of several components. These components include nucleic acid purification, plasmid design, formulation of the delivery vehicle, administration route and schedule, dosing, ...