The sequences of events regulating thrombin generation during tissue factor-initiated clotting in whole blood at 37°C in which the contact pathway was suppressed with corn trypsin inhibitor are studied using quantitative Western blotting of factor V, prothrombin, platelet factor 4, antithrombin Ill, and fibrinogen. In addition, fibrinopeptide A (FPA), thrombin-antithrombin 111 (TAT) complex for...

BLOOD has anomalous rheological properties, particularly in certain disease states. It may therefore be necessary to consider these properties when attempting to assess and perhaps to improve the state of a patient's cerebral circulation. So what evidence is there that cerebral blood flow (CBF) is affected by viscosity? Before attempting to answer this question, one should be aware of what infl...

BACKGROUND AND OBJECTIVES In a previous study we established a reliable setpoint for the prevalence of bacteria in whole blood. In the present study we investigated the possible preventive effect, of diversion of the first 10 ml of a blood donation, on the bacterial contamination rate. MATERIALS AND METHODS To divert the first 10 ml of a whole-blood donation, we used a special five-bag system...

Background: Brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including Iran. Diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. Rapid detection of Brucella species by an automated blood culture system and Polymerase Chain Reaction (PCR) may ...

The sequences of events regulating thrombin generation during tissue factor-initiated clotting in whole blood at 37 degrees C in which the contact pathway was suppressed with corn trypsin inhibitor are studied using quantitative Western blotting of factor V, prothrombin, platelet factor 4, antithrombin III, and fibrinogen. In addition, fibrinopeptide A (FPA), thrombin-antithrombin III (TAT) com...

We evaluated the value of Aspergillus PCR as a tool for diagnosing invasive aspergillosis from whole-blood samples during antifungal therapy. In a 3-year study, 36 patients receiving antifungal therapy due to chest radiographic findings highly suggestive of fungal pneumonia were evaluated. The PCR results from whole-blood samples were compared to those obtained from bronchoalveolar lavage fluid...

umbilical cord blood (ucb) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (hscs). because of insufficient number of cord blood cd34+ cells, the expansion of these cells seems to be important for clinical application. mesenchymal stromal cells (mscs), playing an important role in hscs maintenance, were used as feeder layer. apo...

brucellosis is one of the most common zoonotic diseases in iran and human brucellosis is endemic in all parts of the country. growth of brucella is slow and blood culture of these bacteria by use of classical methods is time-consuming. furthermore, in endemic area culture is required for definitive diagnosis. in the present study, direct urease test and acridine orange staining were tried on th...

A prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. A total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were re...