نتایج جستجو برای: xbai
تعداد نتایج: 718 فیلتر نتایج به سال:
The present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza A M2-protein (M2e), which was fused to the N-terminus of the truncated HSP70 (HSP70359–610) molecule as a new approach for future vaccine research against influenza A. The amplified fragments, M2e and HSP70359-610 genes, were gel-purified. The products were then single digested with Bam...
Purpose: Estrogen as a crucial hormone during pregnancy acts by two types of receptors. Estrogen receptor alpha, expressed by estrogen receptor 1 (ESR1) gene, is more abundant and exists in all human reproductive systems. Association of ESR1 gene polymorphism has been shown in some reproductive fields such as spontaneous abortion, endometriosis-related infertility and in vitro fertili...
We have used pulsed-field gel electrophoresis to derive a restriction map of the chromosome of Bordetella pertussis for the enzymes XbaI, SpeI, PacI, and PmeI, which cleave 25, 16, 2, and 1 times, respectively. The apparent size of the genome is 3,750 kb. The positions of genes for major virulence determinants in the vir regulon and of some housekeeping genes were determined. Apart from the pre...
YUCHUN TANG Research Center of Sectional and Imaging Anatomy Shandong University School of Medicine 44#, Wenhua Xi Road, Jinan, Shandong 250012, China [email protected] XIANG BAI* Department of Electronics and Information Engineering Huazhong University of Science and Technology NO. 1037, Luoyu Road, Wuhan, Hubei 430074, China [email protected] XINGWEI YANG Department of Computer and Informati...
Introduction: Epithelial cell adhesion molecule (EpCAM) is highly expressed on epithelial tumors. So, EpCAM is a valuable antigen for targeted therapy. Using monoclonal antibodies (mabs) is an attractive approach for targeted cancer therapy. Importantly, limitations of intact mabs including large size led to the development of antibody fragments such as single chain fragment variable (scfv). Pi...
Introduction: Epithelial cell adhesion molecule (EpCAM) is a membrane glycoprotein that is overexpressed on the majority of tumor cells of epithelial origin and thereby can be used as a target of immunodetection and immunotherapy of cancer. So, it is important to produce this protein in its native form. Interestingly, during the last two decades, the yeast Pichia pastoris (P. pastoris) has beco...
BACKGROUND Respiratory infection with Pseudomonas aeruginosa is very common in patients with cystic fibrosis (CF) but antimicrobial resistance rates of CF isolates across the UK are largely unknown. METHODS The susceptibility of 417 CF patient isolates of P aeruginosa from 17 hospitals to six commonly prescribed antibiotics were examined. Isolates were tested by an agar break point dilution m...
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