Combining fluorescent zinc sensors with the facile syntheses and biological targeting capabilities of peptides, we created green- and blue-emitting probes that, (i) are readily prepared on the solid-phase, (ii) retain the photophysical and zinc-binding properties of the parent sensor, and (iii) can be directed to the extracellular side of plasma membranes in live cells for detection of mobile z...

All type I DnaJ (Hsp40) homologues share the presence of two highly conserved zinc centers. To elucidate their function, we constructed DnaJ mutants that separately replaced cysteines of either zinc center I or zinc center II with serine residues. We found that in the absence of zinc center I, the autonomous, DnaK-independent chaperone activity of DnaJ is dramatically reduced. Surprisingly, thi...

A zinc(II) complex having planar tridentate ligand, bzimpy, where bzimpy is 2,6-bis(benzimidazol-2-yl) pyridine was synthesized and characterized by UV, NMR, infrared spectroscopy and fluorescence spectra. The zinc complex acts as dibasic acids, in which N-H protons on benzimidazole moieties are responsible for a deprotonation site. Both the absorption spectra and reduction potentials are stron...

Zinc is one of the most widespread metal ions found in biological systems. Of the expected 3000 zinc proteins in the human proteome, most contain zinc in structural sites. Among these structures, the most important are zinc fingers, which are well suited to facilitate interactions with DNA, RNA, proteins and lipid molecules. Knowledge regarding their stability is a critical issue in understandi...

Gold nanoparticles functionalized with imidazolylporphyrinatozinc(II)s were bridged by successive imidazole-to-zinc coordination of bidirectional porphyrinatozinc(II) units.

An unusual beta-elimination reaction involving zinc(II) and LiCl is reported. LiCl and a coordinatively saturated disilazido zinc compound form an adduct that contains activated SiH moieties. In THF/toluene mixtures, this adduct is transformed into a zinc hydride and 0.5 equiv. cyclodisilazane. The Li(+) and Cl(-) ions apparently affect the reaction pathway of the disilazido zinc in a synergist...

In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor, a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At s...

The Escherichia coli zitB gene encodes a Zn(II) transporter belonging to the cation diffusion facilitator family. ZitB is specifically induced by zinc. ZitB expression on a plasmid rendered zntA-disrupted E. coli cells more resistant to zinc, and the cells exhibited reduced accumulation of (65)Zn, suggesting ZitB-mediated efflux of zinc.

3-Hydroxybenzylaminobenzoic acid synthesized in the laboratory as a reagent for the determination of zinc in various water samples. Zinc(II) reacts with 3-hydroxybenzylaminobenzoic acid and forms a light yellow colored complex at pH 5.0. The complex has a maximum absorbance at 460 nm. The complex is stable for more than twelve hours. Hence, a detailed study of the extraction of zinc(II) with 3-...

The selective cleavage of the strong and poorly reactive Si–O–Si bond in functionalized siloxanes under mild conditions is a decisive task for modern synthetic chemistry. Simple treatment of the aminomethyl-functionalized disiloxanes 1, 6, (R,R)-7 and 8 ([R2(CH2NR′)SiO]2, R = Me or Ph, NR′ = NC5H10, NC5H8(CH3)2 or NC4H7(CH3)) with zinc(II) bromide and zinc(II) chloride, respectively, results in...