نتایج جستجو برای: zymogram

تعداد نتایج: 296  

2015
Shilja Choyam Dhanashree Lokesh Bettadaiah Bheemakere Kempaiah Rajagopal Kammara

The generation of a zone of inhibition on a solid substrate indicates the bioactivity of antimicrobial peptides such as bacteriocin and enterocin. The indicator strain plays a significant role in bacteriocin assays. Other characteristics of bacteriocins, such as their dispersal ability and the different zymogram components, also affect bacteriocin assays. However, universal well diffusion assay...

Journal: :Journal of basic microbiology 2008
P Charles V Devanathan Periasamy Anbu M N Ponnuswamy P T Kalaichelvan Byung-Ki Hur

Aspergillus nidulans is a highly potent fungus used in the production of alkaline protease. Extracellular alkaline protease was purified from A. nidulans in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-100 column chromatography. The molecular mass of the enzyme was determined to be 42 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelati...

2011
B. Ravindran A. Ganesh Kumar P. S. Aruna Bhavani Ganesan Sekaran

Animal fleshing, the major proteinaceous solid waste generated from leather industry, was used as substrate for the production of alkaline protease by Bacillus cereus 1173900 in solid-state fermentation (SSF). Maximum protease activity of 12,310 U/g was observed at 60 h in SSF crude extract. The extracted protease enzyme was purified by 53.64-fold through ammonium sulphate precipitation and chr...

2016
Gastón Ezequiel Ortiz Diego Gabriel Noseda María Clara Ponce Mora Matías Nicolás Recupero Martín Blasco Edgardo Albertó

A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease a...

2012
Vibeke Simonsen

Electrophoresis as such has been applied for many purposes, among these, analysis of isozymes or isoenzymes. As the designation indicates, this is a group of enzymes having the same catalytic ability, i.e. they catalyse the same chemical reaction. Due to the fact that enzymes are proteins and built from amino acids, they possess an electric change so isozymes are enzymes with the same catalytic...

Journal: :Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology 1988
G Barnert A Hassl H Aspöck

Zymogram analysis using isoelectric focusing on polyacrylamide gels was performed to characterize and distinguish two Toxoplasma gondii isolates ("strains" BK and RH). The activity of the following 14 enzymes in the cell lysates was investigated: IDH, MDH, ME, 6PG, G6P, LDH, IPO, HEX, PGM, EST, ALP, ACP, LAP, and PGI. Nine enzymes (IDH, G6P, LDH, HEX, PGM, EST, ALP, ACP, and PGI) showed distinc...

Journal: :Latin American Journal of Aquatic Research 2021

Pacific red snapper (Lutjanus peru) is an important commercial species in Mexico with great aquaculture potential; however, digestive physiology still unknown. Therefore, the objective of present work was to characterize proteases L. peru juvenile using biochemical and electrophoretic techniques. Results showed a higher acid protease activity than alkaline proteases, trypsin, chymotrypsin, leuc...

Journal: :iranian journal of biotechnology 2008
sara seifzadeh reza hassan sajedi reyhaneh sariri

thermophilic bacillus sp. gus1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and zymogram analysis. the enzymes were stable in the alkaline ph range (8.0-12.0), with the optimum temperature and ph range of the proteases being 70ºc and 6.0-12.0, respectively. all th...

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