Fusion proteins between a receptor and a pertussis toxin-insensitive G(i)alpha subunit were used to gain insight into the molecular interactions that take place upon mu and delta opioid receptor heterodimerization. When mu opioid receptor-G(i1)alpha fusions were coexpressed with nonfused delta opioid receptors in human embryonic kidney 293 cells, or vice versa, receptor heterodimers were detect...

Let i1 ≥ i2 ≥ i3 ≥ 1 be integers. An L(i1, i2, i3)-labelling of a graph G = (V, E) is a mapping φ : V → {0, 1, 2, . . .} such that |φ(u)− φ(v)| ≥ it for any u, v ∈ V with d(u, v) = t, t = 1, 2, 3, where d(u, v) is the distance in G between u and v. The integer φ(v) is called the label assigned to v under φ, and the difference between the largest and the smallest labels is called the span of φ. ...

The oxidation of Fe(I1) by serum was studied at pH 7.35 and at various oxygen concentrations which approach the physiological conditions of human serum. The nonenzymic oxidation of Fe(I1) was estimated to be insufficient to account for a rate of Fe(III)-transferrin formation necessary to provide an adequate iron supply for hemoglobin and other biosyntheses if Fe(I1) is a relevant source of seru...

The effect of histamine on steady-state mRNA levels of three soluble secretory proteins from chromaffin granules was investigated in cultured bovine adrenal medullary cells. Histamine stimulated secretogranin I1 mRNA 4-fold, with no or only slight effects on chromogranin A and B transcription. After an initial lag phase, secretogranin I1 mRNA increased markedly between 4 and 12 h followed by a ...

appear which matched those observed by Sanchez and Spiro. Figure 9 shows the time development of the observed Raman spectrum at -0.8 V which at long times became the spectrum of high-spin Fe(I1). The time development of the spectroscopic changes at -0.8 V contrasts the instantaneous changes observed in the reduction of adsorbed hemin and instead tracks the slow reduction of the entire solution ...

To identify domains in the dynein heavy chain (Dhc) required for the assembly of an inner arm dynein, we characterized a new motility mutant (ida2-6) obtained by insertional mutagenesis. ida2-6 axonemes lack the polypeptides associated with the I1 inner arm complex. Recovery of genomic DNA flanking the mutation indicates that the defects are caused by plasmid insertion into the Dhc10 transcript...

Recently, in vitro selection using mRNA display was used to identify a novel peptide sequence that binds with high affinity to Galpha(i1). The peptide was minimized to a 9-residue sequence (R6A-1) that retains high affinity and specificity for the GDP-bound state of Galpha(i1) and acts as a guanine nucleotide dissociation inhibitor (GDI). Here we demonstrate that the R6A-1 peptide interacts wit...

ion [2] [3] PrepareMeal(*I1) Kautz Plan Recognition 09/09/97 page 22 Abstraction [3] [4] End(*I1) Although the recognized plan is not fully specified, enough is known to allow the observer to make predictions about future actions of the agent. For example, the observer can predict that the agent will boil water:ion [3] [4] End(*I1) Although the recognized plan is not fully specified, enough is ...