The goal of this study was to reassess serum alkaline phosphatase (ALP) as tumor marker in osteosarcoma. We retrospectively examined serum ALP levels at diagnosis, every therapeutic step (neoadjuvant chemotherapy, surgery, and adjuvant chemotherapy), metastasis, and follow-up and analyzed the role of ALP as tumor marker in 210 osteosarcomas. The diagnostic performances of ALP were validated wit...

We have prepared murine monoclonal antibodies against isolated human bone alkaline phosphatase (ALP, EC 3.1.3.1). Hybridoma supernates were separately screened for reactivity against both human liver and bone ALP. Although most antibody-positive hybrids showed similar reactivity against both isoenzymes, one hybridoma produced an antibody that interacted preferentially with liver ALP. This antib...

We found significant increases in ALP and ALP isoform band 10 in the serum of patients with early insulin-dependent diabetes, rheumatoid arthritis, and in those with multiple sclerosis during periods of disease exacerbation as compared with healthy controls. The ALP isoforms were assayed by isoelectric focusing. Our data suggest that the increase in ALP and ALP-10 closely reflects the abnormal ...

We have determined the effect of diabetes mellitus and starvation/re-feeding on serum alkaline phosphatase (ALP) isoenzymes in the rat. Both ketotic (DKA) and nonketotic (NKDM) diabetic rats showed elevated total ALP activity, primarily due to an increase in intestinal and bone/liver ALP isoenzyme in the NKDM and DKA groups, respectively. There was a decrease in intestinal ALP activity in 3 day...

AIMS To consider a possible genetic origin for the persistent unexplained increase in plasma alkaline phosphatase (ALP) in five non-related patients referred over an 18 month period. METHODS Plasma ALP isoenzyme activities were measured in patients and their first degree relatives. RESULTS In each patient there was a noticeable increase in intestinal plasma ALP, either alone or accompanied ...

We have studied five patients who have exhibited an unusual alkaline phosphatase isoenzyme (ALP EC 3.1.3.1) migrating in an ultrafast position electrophoretically on cellulose acetate. This ALP isoenzyme has been identified in patients with benign and malignant liver diseases. In addition, a number of these patients exhibited a regular ALP liver isoenzyme and a fast (preliver) ALP isoenzyme in ...

Alkaline phosphatises (ALP) are a group of isoenzymes produced by liver (isoenzymes ALP1-1), bones (isoenzymes ALP2), kidneys, small intestine and placenta (isoenzymes ALP-3) [1,2]. The serum ALP level represents the total amounts of isoenzymes that are released from the tissues into the bloodstream. Placenta ALP is physiologically produced by placenta at the brush border membranes of the syncy...

BACKGROUND/AIMS Serum alkaline phosphatase (ALP) levels tend to increase after hepatectomy, however, no previous examinations have yet focused on the relationship between liver regeneration and the individual ALP isoenzymes levels. METHODOLOGY Forty living liver transplantation donors who underwent hemi-hepatectomy were herein investigated. We evaluated the serum ALP levels and ALP isoenzyme ...