Exposure to chemicals such as benzo[a]pyrene (B[a]P) can generate intracellular toxic mechanisms. Fourier-transform infrared (FTIR) spectroscopy is a novel approach that allows the non-destructive analysis of underlying chemical bond alterations in patho-physiological processes. This study set out to examine whether B[a]P-induced whole cell alterations could be distinguished from effects on nuc...

Previously, we developed a reversed-phase HPLC method compatible to high performance liquid chromatography diffuse reflectance Fourier-transform infrared (HPLC-FTIR) thermospray interface for the analysis of dyes. Dye separation achieved with a mixed-mode (SCX-ODS) column using a small gradient (90 to 80% water with acetic acid) and pH 3.25; 10 to 20% acetonitrile was considered to be suitable ...

Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) qualitatively and quantitatively measured resistant starch (RS) in rat cecal contents. Fisher 344 rats were fed diets of 55% (w/w, dry basis) starch for 8 weeks. Cecal contents were collected from sacrificed rats. A corn starch control was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemi...

FTIR spectroscopy has long been used for the analysis of art and historical objects in support of efforts to conserve, restore and validate authenticity of these rare objects. The value of the technique for this application lies in its inherent sensitivity, specificity and non-destructive capabilities. Typical applications include analysis of paint pigments and binders, lacquers and finishes. F...

Monte Carlo (MC) Simulations, Differential Scanning Calorimetry (DSC) and Fourier Transform InfraRed (FTIR) spectroscopy were used to study the melting behavior of single lipid components in two-component membranes of 1,2Dimyristoyl-D54-sn-Glycero-3-Phosphocholine (DMPC-d54) and 1,2-Distearoyl-sn-Glycero-3-Phosphocholine (DSPC). Microscopic information on the temperature dependent melting of th...

The aim of this study was to compare polymerization lamps of various technologies (halogen, high performance halogen, xenon, i.e. plasma arc, as well as diode lamps) to determine the degree of polymerization of composite probes using Fourier-transform infrared (FTIR) spectroscopy. To observe the light-induced curing reaction under a bracket, an attenuated total reflection (ATR)-FTIR was used. T...

Wehave been developing techniques to apply propagating surface plasmon polariton (SPP) resonances to the spectroscopic study of individual, subwavelength-sized particles. SPPs, mixed states of light and conducting electrons at the surface of a patterned metal surface, are part of, that is, mediate, the extraordinary transmission/reflection resonances of patterned metal meshes. A fair body of wo...

Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its app...