The impact of ozone dose and time ozonization on the selected physicochemical properties Agaricus bisporus stored for 14 days in 2 °C was investigated. Mushrooms were subjected to gaseous with increasing concentrations 0.5, 1.0, 2.0 mg L−1 applied two times ozonization: 30 60 min. following parameters evaluated: weight loss, internal external color L*a*b* parameters, overall change (ΔE), browni...

Nucleic acid extraction is an attractive alternative to labor-intensive manual methods. In this report, a few methods of total RNA Extraction from Edible mushroom (Agaricus bisporus) compared. This methods consist AGPC(Acid Guanidinium-Phenol-Chloroform), The method according by Lithium chloride and RNx total RNA isolation Kit (CinnaGen). Best method selected for the preparation of high quality...

Cloned homobasidiomycete TRP2 genes for Agaricus bisporus and Coprinus bilanatus were sequence-characterised. Both genes encode tri-functional proteins with activity domains for glutamine amidotransferase (GAT; G domain), indole glycerol phosphate synthase (InGP; C domain) and phosphoribosyl anthranilate isomerase (F domain). A conserved intron disrupts the GAT-coding sequence in both genes. Co...

Two strains of Agaricus bisporus have been investigated by fluorescent microscopy and scanning electron microscopy. Somatic nuclei, stained with auramin O and examined by fluorescent microscopy, appear to be randomly distributed, divide asynchronously, and assume a nonclassical or "two-track" configuration during mitotic metaphase. A similar configuration has been observed for nuclei during mei...

In wheat straw based composting, enabling growth of Agaricus bisporus mushrooms, it is unknown to which extent the carbohydrate-lignin matrix changes and how much is metabolized. In this paper we report yields and remaining structures of the major components. During the Phase II of composting 50% of both xylan and cellulose were metabolized by microbial activity, while lignin structures were un...

Fungi are vital to numerous industrial and household processes, especially producing cheeses, beer, wine, bread, they accountable for breaking down organic matter. The remarkable medicinal nutritional values of the mushrooms have increased their consumption. Agaricus bisporus belongs Agaricaceae family, it is a top-ranked cultivated mushroom that well known its edibility. A. rich in nutrients s...

The research was carried out in a mushroom farm, College of Agriculture, Tikrit University, five microelements were tested, namely, iron, manganese, molybdenum, Zinc and boron, combination these elements, addition biological agent, Pseudomonas fluva, to control the brown blotch disease caused by pathogenic bacteria tolaasii on edible Agaricus bisporus. results showed that all treatments micro-e...