The previously observed (Walter, et al. 1981 1. Cell Biol. 91 :545-550) inhibitory effect of SRP selectively on the cell-free translation of mRNA for secretory protein (preprolactin) was shown here to be caused by a signal sequence-induced and site-specific arrest in polypeptide chain elongation. The M r of the SRP-arrested nascent preprolactin chain was estimated to be 8,000 corresponding to-7...

Four implicit potential energy surfaces (PESs) with specific-reaction-parameters (SRP) are developed and tested for the reaction CH4 + H → CH3 + H2. The first is called MPW60 and is based on the modified Perdew-Wang (mPW) hybrid density-functional method with the percentage of Hartree-Fock (HF) exchange equal to 60%. The other three PESs are constructed with multi-coefficient correlation method...

BACKGROUND The treatment of periodontitis begins with a non-surgical phase that includes scaling and root planing(SRP) and on occasion the use of systemic antibiotics. The goal was to systematically evaluate in systemic healthy adults the effect of the concomitant administration of amoxicillin (amx) and metronidazole (met) adjunctive to SRP compared to SRP alone. METHODS The PubMed-MEDLINE, C...

We have studied the interaction between the signal sequence of nascent preprolactin and the signal recognition particle (SRP) during the initial events in protein translocation across the endoplasmic reticulum membrane. A new method of affinity labeling was used, whereby lysine residues, carrying the photoreactive group 4-(3-trifluoromethyldiazirino) benzoic acid in their side chains, are incor...

introduction: the aims of the present study were: [1] to evaluate whether erbium-doped: yttrium, aluminum and garnet (er: yag) laser could be a prophylactic methods against transient bacteremia during scaling and root planing (srp) [2] to confirm the efficacies of srp with er: yag laser by clinical and microbiologic evaluations. methods: twenty chronic periodontitis subjects were randomly treat...

The signal recognition particle (SRP) directs integral membrane and secretory proteins to the cellular protein translocation machinery during translation. The SRP is an evolutionarily conserved RNA-protein complex whose activities are regulated by GTP hydrolysis. Recent structural investigations of SRP functional domains and interactions provide new insights into the mechanisms of SRP activity ...

We have investigated the role of signal recognition particle (SRP) in the biosynthesis of Sindbis glycoproteins by translating the viral 26S mRNA in a wheat-germ cell-free system. SRP was shown to have no effect on the synthesis or proteolytic processing of the cytoplasmic C protein. In contrast, the membrane integration and the proteolytic processing of the viral glycoproteins PE2 and E1 were ...

For proteins to enter the secretory pathway, the membrane attachment site (M-site) on ribosomes must bind cotranslationally to the Sec61 complex present in the endoplasmic reticulum membrane. The signal recognition particle (SRP) and its receptor (SR) are required for targeting, and the nascent polypeptide associated complex (NAC) prevents inappropriate targeting of nonsecretory nascent chains....