BACKGROUND The INK4a-ARF (CDKN2A) locus on chromosome 9p21 encodes two tumour suppressor proteins, p16(INK4a) and p14(ARF), whose functions are inactivated in many human cancers. AIMS To evaluate p14(ARF) and p16(INK4a) alterations in liver cell adenoma. METHODS After microdissection, DNA from 25 liver cell adenomas and corresponding normal liver tissue were analysed for INK4-ARF inactivati...

The INK4a/ARF locus encodes two different proteins involved in cell cycle control. Both molecules, p16(INK4a) and p19(ARF), inhibit cell cycle progression and have been shown to act as tumor suppressors in a variety of models. Their expression is controlled by separate promoters responding to different stimuli and they therefore show independent transcriptional regulation. We have cloned and ch...

acute renal failure (arf) following cardiac surgery occurs in 1 to 10% of patients. patients who develop arf have higher rates of mortality. this study was undertaken to estimate the role of perioperative variables in predicting of post cardiac surgery arf. we studied a cohort of 398 adult patients who underwent cardiac surgery at our institution from february 2004 to february 2006. adult patie...

BACKGROUND The p14(ARF) protein encoded by the INK4a/ARF locus promotes degradation of the MDM2 protein and thus prevents the MDM2-mediated inhibition of p53. Homozygous deletion of the INK4a/ARF locus is common in human mesothelioma and may result in the loss of p14(ARF) and the inactivation of p53. We designed this study to evaluate the biologic and potential therapeutic roles of p14(ARF) exp...

The purpose of this study was to evaluate cellular immune responsivenes.s in patients with acute rheumatic fever (ARF). Peripheral blood lymphocytes were obtained for culture from patients who had ARF within the past 2-3 months. No patient was receiving steroids at the time of the study. Peripheral lymphocytes were also obtained from normal control individuals. Lymphocyte cultures were maintain...

ARF-BP1 enhances p42 ubiquitination To explore whether ARF-BP1 mediates p42 ubiquitination, we cotransfected GST-p42 and HAubiquitin into HEK293 cells in the presence of ARF-BP1 or MDM2, followed by MG132 treatment. ARF-BP1 strongly stimulated p42 polyubiquitination, whereas MDM2 barely influenced its ubiquitination. MG132 treatment further elevated the ubiquitination effect (Supplemental Figur...

p14(ARF), the alternative product from the human INK4a/ARF locus, antagonizes Hdm2 and mediates p53 activation in response to oncogenic stimuli. An immunohistochemical study of p14(ARF) expression in 74 samples of aggressive B-cell lymphomas was performed, demonstrating an array of different abnormalities. A distinct nucleolar expression pattern was detected in nontumoral tissue and a subset of...

OBJECTIVE Preoperative assessment of risk factors for postoperative acute renal failure (ARF) is a key point in the management of cardiac surgery patients. A reliable evaluation of individual risk permits the efficient application of prophylactic and therapeutic measures to patients with increased risk of ARF. In 2005, Thakar published a scoring system to determine the ARF risk prior to surgery...

The ARF locus is frequently inactivated in human cancer. The oncosuppressor ARF has indeed been described as a general sensor for different situation of cellular stress. We have previously demonstrated that ARF deficiency severely impairs inflammatory responses in vitro and in vivo, establishing a role for ARF in the regulation of innate immunity. The aim of the present work was to get further ...