In order to develop an easy, rapid and efficient protocol for the genetic transformation of tomato (Lycopersicon esculentum Mill.), without involving tissue culturing, ripened fruits of tomato were infiltrated with Agrobacterium harboring plasmid pROKII GUSint AP1. The plasmid contains Arabidopsis early flowering gene AP1, GUS reporter gene and NPTII as a selectable marker gene. Both transient ...

BACKGROUND The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells in the same tissue. On the other hand, the use of reporter genes often fails to accurately detect...

hrp genes are reportedly required for pathogenicity in Pseudomonas syringae pv. syringae (Pss) and other phytopathogenic bacterial species. A subset of these genes encodes a type III secretion system through which virulence factors are thought to be delivered to plant cells. In this study, we sought to better understand the role that hrp genes play in interactions of Pss with its host as they o...

RecA-mediated recombination requires regions of homology between donor and recipient DNA for successful integration. This paper investigates the effect of the relationship between the length of gene-sized inserts (434, 733, 2228 and 2400 bp) and flanking sequence homology (100 - ca. 11 000 bp) on transformation frequency in Acinetobacter baylyi strain BD413. Both insert size and size of the hom...

Abstract Background With regard to the extensive production of genetically modified organisms, scientists focused on safety antibiotic resistance marker (ARM) genes present in GM-crops produced for consumption human or animal. We investigated possible transfer these microflora gastrointestinal tract and blood cells two groups Male Wistar Albino rats fed a transgenic diet containing ARM nptII aa...