The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

Transepithelial fluid transport(/,) and intracellular Na' and CIactivities (aNai , aCl;) were measured in isolated Necturus gallbladders to establish the contribution of different proposed apical membrane entry mechanisms to transepithelial salt transport . In 10 mM HC03-Ringer's, Jv was 13.5 ± 1 .1 ttl cm-'-h, 'and was significantly reduced by a low bicarbonate medium and by addition of amilor...

Our purpose was to study the influence of the stimulation of the cerebroventricular system on some mechanisms related to hydrosaline equilibrium and blood pressure regulation. Renal function and blood pressure (group 1) as well as the baroreceptor reflex (group 2) were studied. In group 1, we measured diuresis, natriuresis, creatinine clearance, lithium clearance, and blood pressure in control ...

1 The regulation of the benzamil (Bz)-insensitive salt taste receptor was 2 investigated by intracellular Ca ([Ca]i), protein kinase C (PKC) and the Ca3 dependent serine-threonine phosphatase, calcineurin (PP2B), by monitoring chorda 4 tympani taste nerve responses to 0.1M NaCl solutions containing Bz (5x10 M) and 5 resiniferatoxin (RTX; 0-10x10 M) in Sprague-Dawley rats and in wildtype (WT) an...

The chromatographic behaviour of 27 different plasma proteins on fractionation of human plasma on immobilized Cibacron Blue F3-GA was studied. The column was eluted by using a three-step procedure. First, a low-molarity buffer (30 mM-H3PO4/Na3PO4, pH 7.0, I0.053) was used, then a linear salt gradient (0-1 M-NaCl in the buffer above) was applied, followed by a wash with two bed volumes of 1.0 M-...

In this paper, we study the liquid-solid coexistence of NaCl-type alkali halides, described by interaction potentials such as Tosi-Fumi (TF), Smith-Dang (SD), and Joung-Cheatham (JC), and compute their melting temperature (T(m)) at 1 bar via three independent routes: (1) liquid/solid direct coexistence, (2) free-energy calculations, and (3) Hamiltonian Gibbs-Duhem integration. The melting point...

Pyruvate kinase, lactate dehydrogenase, and phosphoenol pyruvate were purchased from Sigma. Restriction endonucleases, shrimp alkaline phosphatase (SAP), and T4 polynucleotide kinase were from New England Biolabs. Covalently closed circular χ +-3F3H dsDNA (a pBR322 derivative, containing tandem χ-sequences (Anderson et al., 1999)) was purified using a QIAGEN " Maxi kit ". M13mp7 replicative for...

Rhizobial symbionts were isolated from the surface (0–0.5 M) and phreatic (3.9–5.0 M) root environments of a mature mesquite woodland in the Sonoran Desert of Southern California, and from variable depths (0–12 m) of non-phreatic mesquite ecosystems in the Chihuahuan Desert of New Mexico. They were tested for their ability to tolerate high salinity, and respire NO3 2 as mechanisms of free-livin...

The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 1...

We investigated the effects of hyperosmolality on survival and proliferation of subconfluent cultures of mIMCD3 mouse renal collecting duct cells. High NaCl and/or urea (but not glycerol) reduces the number of viable cells, as measured with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). Raising osmolality from a normal level (300 mosmol/kg) to 550-1,000 mosmol/kg by addin...