Two antigenically similar subtypes of equine herpesvirus 1 (EHV-1) cause disease in horses. A procedure for rapid differentiation of the two EHV-1 subtypes with monoclonal antibodies was developed. Subtype-specific pools of monoclonal antibodies were constructed, characterized, and used in enzyme immunofiltration and indirect immunofluorescence assays to subtype 50 epizootiologically unrelated ...

immunoglobulin e is one of the five classes of immonoglobulins that plays an important role in allergic diseases. production of monoclonal antibodies by a single clonotype against different epitopes of immunoglobulin e has high priority in development of diagnostic kits. in this study, an attempt was made to produce monoclonal antibodies against human immunoglobulin e. balb/c mice were immunize...

Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

introduction: monoclonal antibodies labeled mainly with 99mtc are being widely used as imaging agents in nuclear medicine. recently pr81 was introduced as a new murine anti-muc1 mab against human breast carcinoma. this antibody reacts with the tandem repeat of 20-mer peptide of protein backbone, and is of igg1 class, with an affinity of 2.19×10-8 m-1. due to high specific reactivity, this antib...

the presence of a low level of m protein in the peripheral blood

results isotype of 1f1 clone was determined to be igg1, which reacted with native protein in the western blot experiment and stained 20% of the membrane of neoplastic cells overexpressing her2 with 3+ grade. however, 3l5 clone showed a low reaction (10%) with native her2 in immunohistochemistry. conclusions the results of both western blotting and immunohistochemistry showed that native her2 ca...

we have employed a peptide-based antibody generation protocol for producing antibody against human nestin. using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any n- or o-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. a wide variety of nestin proteins ranging from 140-250 kda w...