In this work, simultaneous isolation of lactoferrin (Lf) and lactoperoxidase (Lp) from defatted bovine colostrum by one-step cation exchange chromatography with SPEC 70 SLS ion-exchange resin was investigated. A RP-HPLC method for Lf and Lp determination was developed and optimized as the following conditions: detection wavelength of 220 nm, flow rate of 1 mL/min and acetonitrile concentration ...

A detailed procedure for the quantitative determination of metanephrine and normetanephrine is given. Epinephrine and norepinephrine are eliminated by selective adsorption on an alumina column. Metanephrine and normetanephrine are isolated with a cation-exchange resin. Fluorescence is developed by a two-step ferricyanide oxidation at two different pH values followed by tautomerization. The conc...

HE INTRODUCTION OF the carboxylic acid cation exchange Amberlite XE-96 (or IRC-50) Azure A resin’ as the medium for hydrogen ion exchange in place of the quininium exchange resin has simplified greatly the determination of gastric acidity without intubation (1). Solvent extraction and fluorimetry are no longer required. The amount of blue dye excreted in the urine is readily measured by visual ...

Microbial reverse electrodialysis cells (MRECs) combine power generation from salinity gradient energy using reverse electrodialysis (RED), with power generation from organic matter using a microbial fuel cell. Waste heat can be used to distill ammonium bicarbonate into high (HC) and low salt concentration (LC) solutions for use in the RED stack, but nitrogen crossover into the anode chamber mu...

AIM To establish the staining characteristics of certain ion exchange resins in histological material, with a view to enabling confident differential identification. METHODS Various histological staining procedures were applied to selected pathological material and prepared agar blocks containing the cation exchange resin calcium polystyrene sulphonate and the anion exchange resin cholestyram...

Heterogeneous catalysis and their application in industrial-scale organic synthesis of fine chemicals are greatly facilitated by catalysis and further on by catalyst recovery and recycles to fits most “principles of green chemistry. In acid media, terpenoids generally undergoes various transformations. The terpenoid transformations include various liquid Bronsted and Lewis Acids, Acid clays, He...

The estimation of urinary catecholamines has assumed clinical significance mainly because of its value in the diagnosis of phaeochromocytoma (Engel and Euler, 1950; Euler, 1951). Since biological methods have obvious limitations for routine work, various chemical methods have been developed (Lund, 1950; Pekkarinen and Pitkanen, 1955; Euler and Floding, 1955a, b). They are based on the same prin...

The estimation of urinary catecholamines has assumed clinical significance mainly because of its value in the diagnosis of phaeochromocytoma (Engel and Euler, 1950; Euler, 1951). Since biological methods have obvious limitations for routine work, various chemical methods have been developed (Lund, 1950; Pekkarinen and Pitkanen, 1955; Euler and Floding, 1955a, b). They are based on the same prin...

The estimation of urinary catecholamines has assumed clinical significance mainly because of its value in the diagnosis of phaeochromocytoma (Engel and Euler, 1950; Euler, 1951). Since biological methods have obvious limitations for routine work, various chemical methods have been developed (Lund, 1950; Pekkarinen and Pitkanen, 1955; Euler and Floding, 1955a, b). They are based on the same prin...