Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the present investigation has been taken up. An efficient protocol was developed for aseptic seed germination and in vitro shoot multiplication from different aseptic seedling explants. 3...

orchids are one of the most popular plants in the world and among them phalaenopsis genus have the most sales in a global market. because of its hard propagation, micropropagation technique has been employed recently. in this study, we tried to find out useful protocol for commercial production through in vitro culture. at first, for seed capsules production, flowers were pollinated with hand i...

The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...

plant tissue culture techniques were used to study the effect of nacl on tropane alkaloids accumulation and proline content of both datura metel and datura stramonium. callus cultures were established for both datura species. however, ms medium supplemented with 1mg/l of both ba and naa was the best for callus production in d. metel, while ms supplemented with 3 mg/l kin and 1 mg/l 2, 4-d was t...

two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40 min, cont...

two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40...

A protocol was developed for callus induction and regeneration in tomato (Lycopersicon esculentum) var. Moneymaker. Hypocotyl and leaf disc explants of tomato were used as a starting material for callus induction. Explants were cultured on MS medium having different concentrations of hormones. Maximum callogenesis from hypocotyls was obtained on MS medium supplemented with IAA (2 mg/l), NAA (2 ...