ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. Depletion of prokaryotic Era inhibits cell division without affecting chromosome segregation. Previously, we isolated ERAL1 protein as one of proteins which were associated with mitochondrial transcription factor A by using immunoprecipitation. In this study, we analys...

Mytella strigata (Hanley, 1843) is an invasive mussel species that has rapidly spread in China recent years. Here, we tested the utility of three mitochondrial gene fragments, COI, 12S, and 16S, two nuclear D1 28S 18S-ITS1, for characterizing levels genetic diversity among within populations using 191 M. specimens collected to aid ongoing efforts identify origin invasion as well molecular studi...

Mitochondrial biogenesis is controlled by signaling networks that relay information to and from the organelles. However, key mitochondrial factors that mediate such pathways and how they contribute to human disease are not understood fully. Here we demonstrate that the rRNA methyltransferase-related human mitochondrial transcription factors B1 and B2 are key downstream effectors of mitochondria...

The present study has identified a new species from the previously monotypic genus Darwiniella Anderson, 1992. Darwiniella angularissp. n. is similar to Darwiniella conjugatum (Darwin, 1854) in external shell morphology and arthropodal characters. Darwiniella conjugatum, however, has a sharper tergal spur and a less obvious adductor plate angle when compared to Darwiniella angularissp. n. Molec...

Since about 1630, the Imperial Palace has been biologically isolated from other habitats by the development and urbanization of Tokyo. We morphologically examined the skulls of the lesser Japanese mole (Mogera imaizumii) from the Imperial Palace and compared them with those from Kanto District, while the sequences of the cytochrome b and 12S rRNA genes were also analyzed to clarify the genetic ...

The presence of legumin-like constituents within the globulin fractions of wheat (Triticum aestivum), rye (Secale cereale) and corn (maize, Zea mays) was demonstrated. Two-dimensional analysis of wheat globulins in the presence and absence of a reducing agent revealed the existence of reducible approximately 60 kDa polypeptides. Western-blot analysis with 125I-labelled antibodies raised against...

Analyses of the Trypanosoma equiperdum (ATCC 30019) maxicircle reveals deletions, duplications and rearrangement compared to T. brucei. The genes for 9S rRNA and 12 proteins are absent. The 12S rRNA and cytochrome oxidase subunit I (COI) genes lack their 3' ends and are adjacent indicating deletion of intervening genes. The remaining two NADH dehydrogenase subunit genes (ND4 and ND5), the ribos...

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...