Partial sequencing of the 16S-23S rDNA intergenic spacer showed two to four genotypes each for Borrelia hermsii and B. turicatae, both relapsing fever agents transmitted by argasid ticks, and for B. miyamotoi and B. lonestari, transmitted by ixodid ticks. Field surveys of Ixodes ticks in Connecticut and Sweden showed limited local diversity for B. miyamotoi.

AIM The aim of this work was to isolate and molecularly identify enterohemorrhagic Escherichia coli (EHEC) O157 in milk and dairy products in Libya, in addition; to clear the accuracy of cultural and biochemical identification as compared with molecular identification by partial sequencing of 16S rDNA for the existing isolates. MATERIALS AND METHODS A total of 108 samples of raw milk (cow, sh...

Molecular Characterization of Intestinal Bacteria in Healthy Cats and a Comparison of the Fecal Bacterial Flora between Healthy Cats and Cats with Inflammatory Bowel Disease (IBD). (August 2008) Lauren Elizabeth Ritchie, B.S., Texas A&M University Co-Chairs of Advisory Committee: Dr. Jan S. Suchodolski Dr. Jörg M. Steiner Past studies characterizing the feline intestinal microflora have used tr...

AIM Rapid detection of H.pylori strains by PCR-Sequencing. METHODS 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI -BLAST software. RESULTS The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be...

The aim of this study was to develop appropriate protocols for flow cytometric (FCM) and 16S rDNA sequencing investigation of the microbiome in a powdered infant formula (PIF) production facility. Twenty swabs were collected from each of the three care zones of a PIF production facility and used for preparing composite samples. For FCM studies, the swabs were washed in 200 mL phosphate buffer s...

We evaluated the performance of 16S and internal transcribed spacer (ITS) region amplification and sequencing of rDNA from clinical specimens, for the respective detection and identification of bacterial and fungal pathogens. Direct rDNA amplification of 16S and ITS targets from clinical samples was performed over a 4-year period and reviewed. All specimens were from sterile sites and submitted...

Bioleaching is the extraction of metals from their ores through the use of microorganisms. Inthis process, the use of native bacteria leads to achieve more yields of metals. So, in the presentstudy, native sulfur-oxidizing bacterium in potentiality of uranium extraction was isolated fromGhachin mine in Iran and identified by partial gene sequencing. For this purpose, the watersamples were colle...

Among the promising bulk chemicals, 1,3-propanediol (1,3-PD) and 2,3-butanediol (2,3-BD) are two bio-based diols, which have a wide range of applications in cosmetic and food industry, as antifreezes, lubricants, fuel additives, as well as in the production of polymers. The possibility to obtain diols from the waste glycerol is topical by two reasons. First, the waste glycerol is received in la...

A case of skin alterations and mortalities associated with Yersinia ruckeri in two summer old common carp after over-wintering a hibernation pond is described the present work. The disease was characterised mainly by erosions around orbital area fish. bacterium cultivated on Columbia agar identified MALDI-TOF, biochemical characteristics sequencing 16s rDNA. Parasitological virological examinat...

The performance of on-site wastewater treatment systems (OWTSs) can be improved by altering digester design and manipulating environmental variables that affect microbial community composition. Community composition potentially assessed using high-throughput DNA sequencing, but the two most common methods sequencing (16S shotgun sequencing) generally yield different taxonomic identification pro...