Aim. The purpose of this study was to determine the response of circulating markers of lipid and protein oxidation following an incremental test to exhaustion before and after 4 weeks of high-intensity interval training performed in the heat. Methods. To address this question, 16 physically active men (age = 23 ± 2 years; body mass = 73 ± 12 kg; height = 173 ± 6 cm; % body fat = 12.5 ± 6 %; bod...

The antioxidant properties of different almond cultivars (cv.), either regional (Casanova, Duro Italiano, Molar, Orelha de Mula and Pegarinhos cv.) or commercial (Ferraduel, Ferranhês, Ferrastar and Guara cv.) were evaluated through several chemical and biochemical assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of beta-carotene bleaching, in...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Collection and processing of human samples 3.2. Reagents and proteins 3.3. Determination of protein carbonyl groups 3.4. Radioimmunoassay 3.5. Hemoglobin measurement 3.6. Thiobarbituric acid-assay 3.7. Statistical analysis 4. Results 4.1. Analysis of urine samples in cohort 1 4.2. Analysis of urine samples in cohort 2 4.3. Plasma markers...

The thiobarbituric acid (TBA) assay for measuring malonaldehyde (MDA) has been applied to many tissues as a simple means of detecting oxidative damage and prostaglandin synthesis. Human neutrophils, cells known to produce prostaglandins and toxic metabolites of oxygen, were studied to determine if this assay could provide a suitable, rapid, screening test to recognise states of metabolic activa...